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Example sentences for: xtt

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Reduction of XTT was monitored similarly as described for DCIP, except we measured the increase in absorbance at 465 nm resulting from reduction of the colorless oxidized dye to an orange product [ 16 ] .
DCIP, for example, couples directly with NADH or NADPH to form DCIPH 2 and NAD +or NADP +, making it an ideal electron transport chain indicator [ 15 ] . XTT has been used to measure microbial respiratory activity in complex natural environments [ 16 ] . Respiratory dyes have been used commercially for many years in the Biolog™ system to help identify pure cultures of microorganisms by their patterns of oxidation of multiple organic substrates [ 17 ] . Biolog™ also has been used to characterize oxidation capabilities of soil microbial communities [ 18 ] . Rodriquez, et al.
In the Viking's gas exchange experiment, water vapor exposed to the Martian soil released O 2 , but possibly only as a result of the activity of powerful soil oxidants that also might have been responsible for the chemical reactivity seen in other Viking biology experiments [ 20 ] . A molecule such as DCIP or XTT might be destroyed by oxidation in the presence of such soils.
Since DCIP and XTT have different redox potentials, and XTT in our work appears to generally be more easily reduced by soil microbial communities, this shows a need to use more than one respiratory dye in this type of experiment.
Figure 5shows results of XTT reduction by the control and the life-positive Boney Basin, Antarctica soil.