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Example sentences for: xmut

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For the HincII B plasmid, Xmut and Ymut mutagenesis primers were used in conjunction with GL2 for PCR amplification.
The mutagensis primers were as follows: for mutC, 5' GCTCGACCT CCA TTC TAA TG T GGG CAG GTG GTT TCA CAG 3'; mutK, 5' CCA TTC TAA TTG GGG C TT TTT GTT TCA CAG GGA GGA AGT 3'; mutE, 5' TTT CAC AGG GA C CTT GTT GAC AGT TCA ACT TCA AAC 3'; Xmut1, 5' CAC AGG GAG GAA GTT CTG TCA TCA ACT TCA AAC AGG 3'; Xmut2, 5' GAG GAA GTT GAC AGT CTA AGA CT A AAC AGG GTT GGC AGT 3'; Xmut3, 5' TGA CAG TTC AAC TTC GTT AGT GGT TGG CAG TGA CAC 3'; Ymut1, 5' TGG CAG TGA CAC AGG TGT ACT CTG CCT GCT TCC CCT 3'; and Ymut2, 5' ACA CAG GCC CAC ACT G AT GCG TTC CCC TTT CCA CCC CTG 3'.
The Xmut2 mutation had a measurable effect (2-fold decrease) on the full level of transcription and the Ymut2 mutation reduced the reporter activity by more than three-fold (figure 5c).
The mutagenesis primers corresponding to each of the five regions, Ets (mutE), κE2 (mutK), CCAAT (mutC), CD5X (Xmut1, 2, and 3), and CD5Y (Ymut1 and 2) were synthesized at the Protein and Nucleic Acid Facility, Stanford University.
The mutC, mutK, mutE, Xmut, and Ymut mutagenesis primers were first PCR amplified with GL2 primer (5' CTT TAT GTT TTT GGC GTC TTC CA 3') using StuI B plasmid as template to generate megaprimers.