Words similar to xho

Example sentences for: xho

How can you use “xho” in a sentence? Here are some example sentences to help you improve your vocabulary:

A 4.3-kb Hin d III- Xho I fragment that hybridized to Npm3 was identified, subcloned and used to screen a human liver cDNA library.
Briefly, amplified and purified prokaryotic control vectors were linearized at the 5' end using Xho I and purified by gel electrophoresis.
The PCR products were gel-purified using low melting temperature agarose and subjected to restriction enzyme digestion with Nde I and Xho I. The same two restriction enzymes were used to digest plasmid pET-24b+ DNA.
A strain expressing HA-Ppt1p was made using the two-step gene replacement strategy [ 39 ] . First, the upstream sequence of PPT1 (-955 to -1) was amplified from yeast genomic DNA by PCR using a 5' primer containing a Kpn I restriction site (5'-gatcGGTACCattagcacggtgccttaacca-3') and a 3' primer containing a Xho I site and HA coding sequence (5'-gatcCTCGAGatcgataccgtcgagctagcgtagtctgggacgtcgtatgggtatcgactcatATTCGAGATATTTGATTATACCAAGGC-3').
β-catenin cDNAs were inserted into the previously described pBS/MoPrP.Xho vector and the orientation verified by sequencing [ 25 ] . The resulting construct was linearized and gel-purified prior to injection into B6/SJL mouse embryos.