Words similar to xho
Example sentences for: xho
How can you use “xho” in a sentence? Here are some example sentences to help you improve your vocabulary:
The hooks were PCR-amplified from the SV800 vector using specific primer pairs SV-ARS-F and SV-ARS-R (Table 2) and cloned into Xho I- Cla I sites of pARS-VN, yielding the vector pARS-TGAC.
In the 3' primer, the sequence from the Xho I restriction site to the HA coding sequence was identical to that present in YCpIF16.
The reverse primer (5'-GATAAA CTCGAG T-TAACGCGCGTGTCGCACCA-3') was designed to introduce an Xho I site ( CTCGAG ) downstream of the stop codon.
The insert of a Dazap1 cDNA clone P21, which encoded the C-terminal portion of DAZAP1 (starting from aa #197), was cloned in-frame into the Eco RI/ Xho I sites of an expression vector pET32b (Novagen, Madison, WI).
The PCR products were gel-purified using low melting temperature agarose and subjected to restriction enzyme digestion with Nde I and Xho I. The same two restriction enzymes were used to digest plasmid pET-24b+ DNA.