Words similar to xho
Example sentences for: xho
How can you use “xho” in a sentence? Here are some example sentences to help you improve your vocabulary:
Inserts were directionally cloned between an Eco RI site (5') and a Xho I site (3'); however, sequencing indicates that ~22% of clones are in reverse orientation.
The 1316 bp Bam HI- Xho I fragment containing HNT2 was ligated to Bam HI and Xho I-cleaved plasmid pRS423 [ 39 ] to generate plasmid pB05.
The hooks were PCR-amplified from the SV800 vector using specific primer pairs SV-ARS-F and SV-ARS-R (Table 2) and cloned into Xho I- Cla I sites of pARS-VN, yielding the vector pARS-TGAC.
The intron-less pce1 +gene was amplified from plasmid pl32-PCE1 [ 12 ] . The PCR products were digested with Xho I and Bam HI and then inserted into the S. pombe expression vector pREP41X ( LEU2 ars1 +) [ 19 ] . The inserts were sequenced to exclude the acquisition of unwanted mutations during the amplification and cloning steps.
A 900 bp Xho1/Xba1 fragment of Hnf3α genomic DNA that included exon 1 and a portion of intron 1 was used as the 5' arm of homology.