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Four TIPs (TIP3;1, 3;2, 4;1, 5;1) include a sequence reminiscent of the consensus at the putative internal phosphorylation site (G/ R-K /R/H-X- S / T -X-X- R /T) but all TIPs lack such a site at their carboxyl terminus.
No such internal site is found among the NIP/SIP groups, but five NIPS (NIP1;1, 1;2, 2;1, 4;1 and 4;2) contain the carboxy-terminal sequence K -X-X- S -X-X- K / R - S / T /R/A.
A site with the sequence R-K -X- S -X-X- R is present at the internal loop in most PIPs.
They identified conserved residues (indicated in bold type below) in a loop following the first NPA motif ( R - K -X- S -X-X- R/K ) and close to the carboxyl terminus ( K-K / X -X-X- S -X- R/K-S ). We analyzed the predicted amino-acid sequences of the Arabidopsis AQPs for such motifs.