Example sentences for: x-g

How can you use “x-g” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The transformants were first streaked onto a master plate, then replicated onto two testing plates: U -H -W -glucose plate with X-Gal and U -H -W -galactose/raffinose plate with X-Gal.

  • Following outgrowth at 37°C for 4 h, transformants were recovered by plating on TSA containing gentamicin and X-Gal.

  • Then, cells were fixed with 0.5% glutaraldehyde and stained for β-galactosidase (LacZ) activity using 5-bromo-4-chloro-3-indoyl-β- D-galactopyronoside (X-Gal) as the substrate.

  • After superinfection, DpnI -resistant (non-methylated; replicated) plasmid DNA was rescued in E. coli . In the presence of X-Gal, bacterial colonies which turn blue do not contain mutations in the LacZ promoter or LacZ coding region.

  • For presentation, yeast transformants were also directly streaked onto U -H -W -galactose/raffinose plate with X-Gal, after confirming lack of the auto-activation on glucose plates.


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