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The fasta_groups.txt file is particularly useful for subsequent Clustal X (Clustal X Help http://www-igbmc.u-strasbg.fr/BioInfo/ClustalX/Top.html; [ 4 5 ] ) and BLAST analyses (BLAST http://www.ncbi.nlm.nih.gov/BLAST/; [ 6 ] ). The second output file, group_seqs.txt, contains the group name and all sequences from the group.