Words similar to wunsch
Example sentences for: wunsch
How can you use “wunsch” in a sentence? Here are some example sentences to help you improve your vocabulary:
Neutrophils were recovered, after digestion of the lung tissue for 25 min with 60 U/ml collagenase D (specific activity 0.22 Wunsch unit = 800 IU, low ancillary protease activity, Boehringer Mannheim, Indianapolis, IN, USA), prepared in HBSS containing 10% fetal bovine serum (FBS) and 0.01% DNAse 1 (Sigma Chemical Co., St. Louis, MO, USA), followed by filtering (220 μm).
The sequences of AChBP, and of the alpha3, alpha4, alpha7, beta2 and beta4 nAChR monomers were aligned using the ICM multiple sequence alignment algorithm that includes the following steps [ 16 10 ] : 1) pairwise alignment using Needleman and Wunsch algorithm modified to use zero end gap penalties (so called ZEGA) [ 15 16 ] . 2) evolutionary tree construction with a "neighbor-joining algorithm" to determine the order of alignment and calculate relative weights of sequences and profiles from the branch lengths [ 17 ] . 3) traverse the tree from top to bottom, aligning the closest sequences or profiles.
The final assemblies of EST and cDNA or mRNA sequences were aligned to the index NT_017021 genomic sequence using the Martinez Needleman-Wunsch algorithm in MegAlign (DNA Star, Lasergene) permitting identification of exon-intron boundaries, new exons (P24), and the direction of transcription relative to the telomere.
SEQUOIA uses a conventional dynamic programming algorithm based on the work of Needleman and Wunsch [ 30] to find the globally optimal alignment given a particular residue comparison matrix and gapping model.
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