Words similar to wu-blastx
Example sentences for: wu-blastx
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WU-BLASTX parameters were E = 10, W = 3, T = 12.
Analyses of the WMi and WMj data were performed via a local installation of WU-BLAST 2.0 [ 48 ] . Each sequence in WMi and WMj was extracted into individual FASTA format files using Perl scripts and submitted for three six-phase translated WU-BLASTX searches, once each against the C. elegans , Drosophila and bacterial protein databases.
Following clustering, comparative analyses were performed using WU-BLASTX and WU-TBLASTX [ 92 93 ] with 1,798 contig consensus sequences (themselves grouped into 1,625 cluster groups) as queries versus multiple databases including SWIR v.21 (5/19/2000) non-redundant protein database and Wormpep v.54 C. elegans protein database (Wellcome Trust Sanger Institute, unpublished work), C. elegans mitochondrial protein sequences, and six internally constructed databases using intersections of data from the GenBank nucleotide database and dbEST [ 96 ] . These include: nemnoele (all nucleotide data from the phylum Nematoda with C. elegans removed); nemnoelenomi (nemnoele with M. incognita removed); nemnoelenomel (nemnoele with all Meloidogyne species removed); nemnoelenotyl (nemnoele with all Tylenchida species removed); yestylnomel (all Tylenchida species except Meloidogyne ); mj (only M. javanica sequences).
WU-BLASTX (translated nucleotide query versus protein database) parameters were S = 100 M = PAM120 V = 0 W = 4 T = 17.