Words similar to wst-

Example sentences for: wst-

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Cells were then returned into the incubator for 40-45 hrs before the addition of 1/10 vol of WST-1.
Plates were incubated in a humidified 5% CO 2 incubator at 36°C and the reduction of the WST-1 reagent to formazan was measured repeatedly after the addition of the WST-1 reagent, at either 415 nM (neurons) or 450 nM (microglia), with a microtiter plate (ELISA) reader.
A colorimetric assay for the quantitation of cell viability, based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]1,3-benzene disulfonate) (slightly red) to formazan (red) by mitochondrial dehydrogenases in viable cells was utilized for these studies.
Domoic acid has been reported to be neurotoxic to neuronal tissue in vitro in concentrations ranging from 10 μM to 20 mM [ 28 29 30 31 32 33 ] . In order to determine a concentration of domoic acid that was neurotoxic in vitro during a 6 hour exposure, we investigated the concentration-dependent effect of domoic acid on cerebellar granule neurons using the WST-1 viability assay.
This assay measures cell viability as a function of cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells to formazan.