Words similar to wst-
Example sentences for: wst-
How can you use “wst-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were then returned into the incubator for 40-45 hrs before the addition of 1/10 vol of WST-1.
The cytotoxicity of domoic acid to neuronal tissue in vitro has been investigated over a wide range of concentrations, namely at 10 μM with primary cultures of cerebellar neurons [ 30 31 ] as well as hippocampal slices [ 33 ] , at 100 μM with cultured prenatal rat hippocampal neurons [ 29 ] and at 20 mM with rat lumbar spinal interneurons [ 28 ] . In order to determine a concentration of domoic acid for our in vitro studies that was toxic to cerebellar granule cells after a short-term exposure in our experimental conditions, we completed a viability study using the WST-1 method.
A colorimetric assay for the quantitation of cell viability, based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]1,3-benzene disulfonate) (slightly red) to formazan (red) by mitochondrial dehydrogenases in viable cells was utilized for these studies.
Domoic acid has been reported to be neurotoxic to neuronal tissue in vitro in concentrations ranging from 10 μM to 20 mM [ 28 29 30 31 32 33 ] . In order to determine a concentration of domoic acid that was neurotoxic in vitro during a 6 hour exposure, we investigated the concentration-dependent effect of domoic acid on cerebellar granule neurons using the WST-1 viability assay.
Plates were incubated in a humidified 5% CO 2 incubator at 36°C and the reduction of the WST-1 reagent to formazan was measured repeatedly after the addition of the WST-1 reagent, at either 415 nM (neurons) or 450 nM (microglia), with a microtiter plate (ELISA) reader.
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