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Example sentences for: willebrand
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Cell identity and purity was determined as previously described [ 10 ] : all cells stained (by immunofluorescence) negative for von Willebrand factor, PECAM and anti-myosin, but were positive for cytokeratin.
The ability of a MIDAS protein to bind its protein ligand is tightly linked with the presence of intact MIDAS motifs: mutations to the metal-coordinating residues in these motifs, weaken or eliminate ligand binding [ 35 36 ] . An additional set of MIDAS-like proteins, that includes the integrin β-chain [ 37 38 ] and the von Willebrand Factor A domain [ 27 ] , lacks one of the three motifs and appears to bind ligands in a somewhat different manner [ 27 39 40 ] . In midasin, the presence of all three MIDAS motifs in the M-domain, with the putative metal ion-binding residues in the motifs invariant in the available organisms, indicates that midasin belongs to the family of MIDAS-containing proteins.
The second platelet GP of interest is GPIbα, a transmembranous platelet GP (molecular weight, 143,000) that forms noncovalent complexes with GPIbβ, GPIX, and GPV to form the GPIb/IX/V receptor, which is involved in shear stress-induced platelet activation by binding to von Willebrand factor (vWF).
The identity and purity of these cultures was established by immunofluorescent staining as previously described [ 9 ] (in addition to the extensive characterization by Clonetics, we have determined that these cells lack von Willebrand factor and platelet endothelial cell adhesion molecules (PECAM), but are positive for cytokeratin, immunofluorescence and alkaline phosphatase activity).
Endothelin-1 is a vascular hormone, which may play a role in the generation and maintenance of heart failure [ 18 ] . In hypertensive patients, doxazosin lowers von Willebrand factor commensurate with blood pressure, but it does not lower high levels of endothelin-1 while atenolol does [ 19 ] .
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