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In th*/e "Matrix of All Possible Helices" plot http://www.rna.icmb.utexas.edu/CSI/2STR/, the same RNA sequence is extended along the X- and Y-axes, with all potential helices that are comprised of at least four consecutive Watson-Crick (G:C and A:U) or G:U base pairs shown below the diagonal line.
This appears inconsistent with the revertant sequences here, which show selection for a Watson-Crick base pair at the position 3' but not 5' to the branch-point nucleotide.
The angle of the vector corresponds to the mean phase for the group of points, and the magnitude of the vector represents the variability in the phase estimates [ 32 ] . The Watson-Williams-Stevens statistic was applied to evaluate whether two such vectors are significantly different from one another [ 32 ] . Significant differences were evident in the phase of these two clock genes' cyclical expression under a light-dark cycle, for all tissues except isolated antennal pairs and the heads (see Figure 7) and 8.
In this case two average vectors are compared, and an F-statistic (Watson-Williams-Stevens test in [ 63 ] ) is calculated to test whether the means are drawn from the same population or not.
More recent support for this upstream weak or non pairing with simultaneous downstream pairing, was observed in all lariat forming group II introns in barley chloroplasts [ 23 ] . Here 13/16 introns lacked Watson-Crick pairing above the branch-point A and G:C was the downstream base pair whenever there was an upstream A:C, A:G or G:G.
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