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In th*/e "Matrix of All Possible Helices" plot http://www.rna.icmb.utexas.edu/CSI/2STR/, the same RNA sequence is extended along the X- and Y-axes, with all potential helices that are comprised of at least four consecutive Watson-Crick (G:C and A:U) or G:U base pairs shown below the diagonal line.
The angle of the vector corresponds to the mean phase for the group of points, and the magnitude of the vector represents the variability in the phase estimates [ 32 ] . The Watson-Williams-Stevens statistic was applied to evaluate whether two such vectors are significantly different from one another [ 32 ] . Significant differences were evident in the phase of these two clock genes' cyclical expression under a light-dark cycle, for all tissues except isolated antennal pairs and the heads (see Figure 7) and 8.
More recent support for this upstream weak or non pairing with simultaneous downstream pairing, was observed in all lariat forming group II introns in barley chloroplasts [ 23 ] . Here 13/16 introns lacked Watson-Crick pairing above the branch-point A and G:C was the downstream base pair whenever there was an upstream A:C, A:G or G:G.
Upon Watson-Crick base pairing, the proximity of the synthetic reactive groups elevates their effective molarity by several orders of magnitude, inducing a chemical reaction.
The Watson-Williams-Stevens test returns an F-statistic for the comparison of vectors between two groups to determine whether they represent significantly different estimates of phase (see Figures 5, 6and [ 27 32 ] for further detail).
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