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In th*/e "Matrix of All Possible Helices" plot http://www.rna.icmb.utexas.edu/CSI/2STR/, the same RNA sequence is extended along the X- and Y-axes, with all potential helices that are comprised of at least four consecutive Watson-Crick (G:C and A:U) or G:U base pairs shown below the diagonal line.
For double-stranded DNA molecules, this is necessarily true because of Watson-Crick base pairing.
Additionally, no barley intron was found, in which the upstream Watson-Crick pair coincided with a downstream G:U or other weak pair.
This appears inconsistent with the revertant sequences here, which show selection for a Watson-Crick base pair at the position 3' but not 5' to the branch-point nucleotide.
In this case two average vectors are compared, and an F-statistic (Watson-Williams-Stevens test in [ 63 ] ) is calculated to test whether the means are drawn from the same population or not.