Words similar to vortexing
Example sentences for: vortexing
How can you use “vortexing” in a sentence? Here are some example sentences to help you improve your vocabulary:
After washing twice in PBS, cells were resuspended in ice-cold methanol with vortexing, then allow to sit for 15 min on ice.
The total protein was extracted from approximately 10 7cells by vortexing with glass beads, then resolved by SDS-PAGE on 12% gel, and transferred to nitrocellulose at 55V in (25 mM Tris, 250 mM glycine, 0.1% SDS, and 20% methanol) for 1 hr at room temperature.
Samples were mixed by vortexing and allowed to stand at room temperature for 2-5 minutes more, then centrifuged for 15 min at 10,000 g . The upper aqueous layer was removed to a clean tube and RNA was precipitated with an equal volume of isopropanol.
Cells were pelleted at 400 g for five minutes without brakes, the supernatant was decanted and the pellet was loosened by vortexing at setting 4. Cells were washed with 1% BSA/PBS and repelleted.
Cumulus cells were removed by vortexing with 0.1% (w:v) hyaluronidase and denuded oocytes were transferred into 100 μl of Hepes-buffered TCM-199 supplemented with 7.5 μg/ml cytochalasin B (Sigma) and 10% FCS with the osmolarity adjusted to 300 mOsm by adding sorbitol (manipulation medium) at 29 or 42 h after IVM.
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