Example sentences for: vortexing

How can you use “vortexing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing twice in PBS, cells were resuspended in ice-cold methanol with vortexing, then allow to sit for 15 min on ice.

  • The total protein was extracted from approximately 10 7cells by vortexing with glass beads, then resolved by SDS-PAGE on 12% gel, and transferred to nitrocellulose at 55V in (25 mM Tris, 250 mM glycine, 0.1% SDS, and 20% methanol) for 1 hr at room temperature.

  • Samples were mixed by vortexing and allowed to stand at room temperature for 2-5 minutes more, then centrifuged for 15 min at 10,000 g . The upper aqueous layer was removed to a clean tube and RNA was precipitated with an equal volume of isopropanol.

  • Cells were pelleted at 400 g for five minutes without brakes, the supernatant was decanted and the pellet was loosened by vortexing at setting 4. Cells were washed with 1% BSA/PBS and repelleted.

  • Cumulus cells were removed by vortexing with 0.1% (w:v) hyaluronidase and denuded oocytes were transferred into 100 μl of Hepes-buffered TCM-199 supplemented with 7.5 μg/ml cytochalasin B (Sigma) and 10% FCS with the osmolarity adjusted to 300 mOsm by adding sorbitol (manipulation medium) at 29 or 42 h after IVM.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast