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Example sentences for: visualized
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Four-color flow cytometry was performed using the following monoclonal antibodies (Pharmingen): biotin anti-mouse CD25 (clone: 7D4) visualized with Per-CP conjugated streptavidin; APC anti-mouse CD44 (clone: IM7); and three phycoerythrin (PE) conjugated antibodies: anti-mouse CD4 (clone: RM4-5), anti-mouse CD8a (clone: 53-6.
Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
Through a midline abdominal incision the infra-renal abdominal aorta and the inferior vena cava were visualized.
The sections were then incubated with 3% normal serum (in 0.01 M phosphate buffer-saline, 0.1 % Tx-100; 30 min) followed by incubation with anti-MIF antibody (1:1600) overnight at 4°C and visualized as described previously [ 11 ] . Briefly, the sections were exposed to a goat anti-rabbit antiserum (Jackson; 1:50 in PBS) for 1 hour, followed by exposure to goat-PAP (Jackson; 1:100 in PBS).
When cultures transfected with Δpre-M/E-gp120 were trypsinized and replated on day 7 post transfection and then analyzed on day 9 post transfection, fluorescent cells were readily visualized (Figure 7).
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