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Example sentences for: visualized

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Visualization of AlexaFluor-647-stained lymphocytes was achieved using a Cy5 filter cube (620 nm excitation, 660 nm long-pass beam splitter dichroic mirror, and 700 nm emission), while AlexaFluor-488-stained lymphocytes were visualized with a fluoroisothiocyanate (FITC) filter cube (480 nm excitation, 505 nm long-pass beam splitter dichroic mirror, and 535 Â± 25 nm emission).
After washing with deionized water and PBS three times, species-specific secondary antibodies were added and the antibody complex was visualized by 3,3'-diaminobenzidine.
When cultures transfected with Δpre-M/E-gp120 were trypsinized and replated on day 7 post transfection and then analyzed on day 9 post transfection, fluorescent cells were readily visualized (Figure 7).
thapsigargin and ryanodine) deplete the ER calcium pool either by (i) inhibiting the ER calcium pump and thereby preventing the replenishment of the ER calcium pool or (ii) triggering ER (ryanodine or inositol 1,4,5-trisphosphate (IP 3 ) activated) calcium release channels [ 9 ] . These phenomena can be visualized by fluorescence microscopy as a transient increase in cytoplasmic calcium concentrations (for example see [ 10 ] ). Nominally calcium-free media are used so that the effect can be distinguished from calcium influx mediated via plasma membrane channels.
After electrophoresis, gels were enhanced by immersion in 2,5-diphenyloxazole (PPO) and visualized by autoradiography.