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Example sentences for: vistra

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The efficiency of the oligonucleotide immobilization strategy was tested by stringently washing the same slide overnight in 0.2% SDS (at 42°C, SSC absent in wash solution) to remove Vistra Green and any loosely bound nucleic acids, restained with Vistra Green and scanned (Figure 1b).
Directly following probe deposition, UV cross-linking at 250 mJ/cm 2and Vistra Green staining, the measured fluorescence intensity from oligonucleotide probes was typically higher than PCR amplicons (Figure 1a).
To assess oligonucleotide retention, slides were UV cross-linked and stained for 10 min in Vistra Green Nucleic Acid staining solution (Amersham Pharmacia, Piscataway, NJ) at a 1:10,000 dilution.
Subsequently, slides were gently agitated in 0.2% SDS (no salt) overnight at 42°C, washed extensively with water, scanned to ensure that the dye was completely removed, restained with Vistra Green and rescanned.
On the basis of the hybridization experiments and in agreement with Vistra Green staining results, optimal attachment of 70-mer oligonucleotides occurred between 250 and 450 mJ/cm 2. An improvement in oligonucleotide retention onto aminated slides was not seen at intensities higher than 450 mJ/cm 2(data not shown).