Words similar to vectastain

Example sentences for: vectastain

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Phospho-ERK was detected by mouse anti-phospho-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, used at 1:1500) and the Vectastain ABC kit (Vector Lab.
For labeling of immunoreactivity with the diaminobenzidine (DAB), the Vectastain Elite ABC-Kit (Vector Laboratories, Burlingame, CA) was used according to the suggested protocol.
After washing 2 × 15 min with PBS + 0.1% Triton, slides were treated with Vectastain Elite reagent (avidin-peroxidase-complex) for 1 - 1½ h at 37°C.
The antibodies were affinity purified against the immunizing peptide (anti-TGF-β 3 ) or against protein A sepharose (anti-TGF-β 1 -LC, anti-TGF-β 1 -CC and anti-TGF-β 2 ). Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit, Vector Laboratories, Burlingame, CA, USA).
Synovial samples that had been examined for chemokine binding were also sectioned and incubated with rabbit polyclonal antibodies to the carboxy termini of CXCR1 and CXCR2 (both from Santa Cruz Biotechnology, Santa Cruz, USA) [ 20 ] . Antibody binding was detected using an immunoperoxidase method (Vectastain ABC Elite Kit; Vector Labs, Burlingame, USA).