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Example sentences for: vectastain

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Synovial samples that had been examined for chemokine binding were also sectioned and incubated with rabbit polyclonal antibodies to the carboxy termini of CXCR1 and CXCR2 (both from Santa Cruz Biotechnology, Santa Cruz, USA) [ 20 ] . Antibody binding was detected using an immunoperoxidase method (Vectastain ABC Elite Kit; Vector Labs, Burlingame, USA).
The antibodies were affinity purified against the immunizing peptide (anti-TGF-β 3 ) or against protein A sepharose (anti-TGF-β 1 -LC, anti-TGF-β 1 -CC and anti-TGF-β 2 ). Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit, Vector Laboratories, Burlingame, CA, USA).
After washing 2 × 15 min with PBS + 0.1% Triton, slides were treated with Vectastain Elite reagent (avidin-peroxidase-complex) for 1 - 1½ h at 37°C.
After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.
Biotinylated mouse Ig, streptavidin/horseradish peroxidase conjugate, diamino benzidine, and Vectastain ABC were from Vector (Burlingame, CA, USA).