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Example sentences for: vectastain

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Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit; Vector Laboratories) following treatment of sections with hyaluronidase to improve antibody penetration.
Embryos were washed four times before being developed using the Vectastain ABC kit Elite (Vector, CA) and diaminobenzidine (Sigma Fast DAB).
In brief, after dewaxing, inactivating endogenous peroxidase activity and blocking cross-reactivity with normal serum (Vectastain Elite Kit; Vector, Burlingame, CA), the sections were incubated overnight at 4□ with a diluted solution of the primary antibodies (1:1000 for p53, 1:200 for iNOS and 1:100 for VEGF).
After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.
For labeling of immunoreactivity with the diaminobenzidine (DAB), the Vectastain Elite ABC-Kit (Vector Laboratories, Burlingame, CA) was used according to the suggested protocol.