Example sentences for: vectastain

How can you use “vectastain” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The primary antibody was visualized with the Vectastain Elite ABC mouse kit (Vector Labs).

  • Paraffin-embedded, and fresh-frozen lung sections (5 μm) were immunostained using monoclonal antibodies against human MMP12 (R&D Systems) or non-immune antisera by an immunoperoxidase protocol (Vectastain Elite, Vector Labs, Burlingame, California, United States) and counterstained with hematoxylin as recommended by the manufacturer.

  • Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit; Vector Laboratories) following treatment of sections with hyaluronidase to improve antibody penetration.

  • The bound antibody was detected using biotinylated second antibody, avidin-peroxidase conjugate and diaminobenzidine substrate (Vectastain ABC Elite Kit; Vector Labs, Burlingame, USA).

  • After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.


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