Words similar to vectastain

Example sentences for: vectastain

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Paraffin-embedded, and fresh-frozen lung sections (5 μm) were immunostained using monoclonal antibodies against human MMP12 (R&D Systems) or non-immune antisera by an immunoperoxidase protocol (Vectastain Elite, Vector Labs, Burlingame, California, United States) and counterstained with hematoxylin as recommended by the manufacturer.
After washing 2 × 15 min with PBS + 0.1% Triton, slides were treated with Vectastain Elite reagent (avidin-peroxidase-complex) for 1 - 1½ h at 37°C.
Embryos were washed four times before being developed using the Vectastain ABC kit Elite (Vector, CA) and diaminobenzidine (Sigma Fast DAB).
The bound antibody was detected using biotinylated second antibody, avidin-peroxidase conjugate and diaminobenzidine substrate (Vectastain ABC Elite Kit; Vector Labs, Burlingame, USA).
In brief, after dewaxing, inactivating endogenous peroxidase activity and blocking cross-reactivity with normal serum (Vectastain Elite Kit; Vector, Burlingame, CA), the sections were incubated overnight at 4□ with a diluted solution of the primary antibodies (1:1000 for p53, 1:200 for iNOS and 1:100 for VEGF).