Example sentences for: vac

How can you use “vac” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The Cy3 (HL60) and Cy5 (HL60/TLK286) PCR products were purified with Qiagen PCR purification kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.

  • The products were then dried in a Savant DNA110 Speed Vac.

  • The labeled DNA was mixed with 50 μl of 100 mM sodium acetate (pH 5.2), purified with Qiagen PCR Purification Kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.

  • The volume was reduced to about 10 μl using a Speed-Vac system (ThermoSavant, AES2010) before hybridization.

  • The resultant solutions were dried at room temperature in the Speed Vac, and the pellets were resuspended in 20 μl 0.1 M Na 2 CO 3 (pH 9.0) and mixed with 10 μl of previously prepared Cy3 or Cy5 dye.


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