Words similar to usf
Example sentences for: usf
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in lipid metabolism [ 39 40 ] , and a possible function of USF in tuning RIα/RIIβ ratio by activating RIα transcription and inhibiting RIIβ transcription is an interesting target of further investigation.
Other truncated USF1 or USF2 isoforms could not be detected in this assay, which may indicate that the levels of isoforms forming complex V (Fig.
C; catalytic subunit, cAMP; cyclic adenosine monophosphate, CAT; chloramphenicol acetyl transferase, C/EBP; CAAT/enhancer binding protein, ChoRE; carbohydrate response element, CMV; cytalomega virus, 8-CPT-cAMP; 8-(4-chlorophenyl)thio-cAMP, CREB; cAMP-response element binding protein, EMSA; electrophoretic mobility shift assay, FAS; fatty acid synthase, FSH; follicle stimulating hormone, GIRE; glucose/insulin response element, hCG; human chorionic gonadotrophin, HLH; helix-loop-helix, IRE; insulin response element, MHC; major histocompability complex, PGHS; prostaglandin G/H synthase, PKA; cAMP-dependent protein kinase, R; regulatory subunit, USF; upstream stimulating factor, WB; Western blot.
Among genes that are stimulated by FSH/cAMP during the period of maturation of Sertoli cells (15 to 20 days of age in rats), are several subunits of cAMP-dependent protein kinase (PKA), the serine/threonine kinase mainly responsible for the downstream effects of FSH [ 15 ] . Expression of the RIIβ regulatory subunit of cAMP-dependent protein kinase is highly induced (50-fold) at the mRNA level in primary cultures of rat Sertoli cells as a late response to cAMP peaking at 12 hours [ 16 ] . We have demonstrated that expression of CAAT/Enhancer binding protein β (C/EBPβ) is induced by cAMP with rapid kinetics in Sertoli cells, and that C/EBPβ is responsible for induction of late response genes like the RIIβ-gene [ 17 ] . The RIIβ promoter contains a conserved E-box/HLH element in the basal promoter localized at -280 to -275 relative to the functional ATG [ 18 ] . This element is shown to be important for cAMP-responsiveness in granulosa cells, and it has been shown to bind both USF and Myc in CHO and NB2a cells [ 18 19 ] . In this work, we show that USF isoforms may regulate cAMP responsiveness of the RIIβ promoter by modulating the effect of C/EBP, and that the formation of USF isoforms may be regulated by cAMP in Sertoli cells.
In response to glucose, transcription of several glycolytic and lipogenic genes are activated through the glucose/carbohydrate/insulin response element (GIRE, ChoRE or IRE) [ 3 4 ] . Upstream stimulatory factors (USF) 1 and 2 are characterized by a basic/helix loop helix/leucine zipper domain responsible for dimerization and DNA binding, and are major components of the GIRE complex [ 5 6 7 8 ] .
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