Words similar to usb
Example sentences for: usb
How can you use “usb” in a sentence? Here are some example sentences to help you improve your vocabulary:
This step is used to verify - by sequencing with the Thermo Sequenase Radiolabeled Termintor Cycle Sequencing Kit (USB) - the composition of a particular band, which can be cut out, eluted and reamplified (not shown), or simply cloned from the P1-7A reaction (see Fig.
PCR products were either purified using ExoSAP-IT (USB Co, Cleveland, OH) or subcloned into a TA cloning vector (Invitrogen, Carlsbad, CA) per the manufacturer's instructions.
DNA obtained from the resultant colonies was sequenced with the USB Sequenase version 2.0 DNA Sequencing kit (Amersham, Cleveland, OH) using T3 and T7 primers.
After amplification, reactions were incubated at 37°C for 15 min with 0.5 Uμl -1exonuclease I (USB) and 0.5 Uμl -1shrimp alkaline phosphatase (Amersham Life Sciences).
Amplification reactions were performed in a total volume of 40 μl and contained 2 μl eluted phage, 40 ng of each primer (FADI 5'-CACTACAATGGATGATG-3' and RADI 5'-CTTGCGGGGTTTTTCAG-3'), 0.001% Tween 20 (Sigma), 2.5 U Taq DNA polymerase (Promega), 1x Taq polymerase buffer (Promega), 1.56 mM MgCl 2 (Promega), and 0.25 mM of each dNTP (USB).