Example sentences for: usb

How can you use “usb” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This step is used to verify - by sequencing with the Thermo Sequenase Radiolabeled Termintor Cycle Sequencing Kit (USB) - the composition of a particular band, which can be cut out, eluted and reamplified (not shown), or simply cloned from the P1-7A reaction (see Fig.

  • PCR products were either purified using ExoSAP-IT (USB Co, Cleveland, OH) or subcloned into a TA cloning vector (Invitrogen, Carlsbad, CA) per the manufacturer's instructions.

  • DNA obtained from the resultant colonies was sequenced with the USB Sequenase version 2.0 DNA Sequencing kit (Amersham, Cleveland, OH) using T3 and T7 primers.

  • After amplification, reactions were incubated at 37°C for 15 min with 0.5 Uμl -1exonuclease I (USB) and 0.5 Uμl -1shrimp alkaline phosphatase (Amersham Life Sciences).

  • Amplification reactions were performed in a total volume of 40 μl and contained 2 μl eluted phage, 40 ng of each primer (FADI 5'-CACTACAATGGATGATG-3' and RADI 5'-CTTGCGGGGTTTTTCAG-3'), 0.001% Tween 20 (Sigma), 2.5 U Taq DNA polymerase (Promega), 1x Taq polymerase buffer (Promega), 1.56 mM MgCl 2 (Promega), and 0.25 mM of each dNTP (USB).


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