Words similar to unsubtracted
Example sentences for: unsubtracted
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One of the main advantages of SSH is that it normalizes the cDNA abundance so that cDNAs encoded by genes that are expressed infrequently, but nonetheless differentially, can be identified readily [ 33 ] . The efficiency of normalization is illustrated in Figure 2. The more uniform distribution of hybridization intensities obtained using the subtracted cDNA probe (Figure 2c) reflects the equalization in the concentrations of individual species present at markedly different concentrations in the initial unsubtracted cDNA populations (Figure 2b).
Binding intensities were quantified using a densitometer and differential gene expression of cloned template was confirmed when binding intensity was 5X greater when probed with labeled subtracted tester cDNA compared to the intensity of subtracted driver cDNA and a greater binding intensity when hybridized with unsubtracted tester cDNA compared to unsubtracted driver cDNA.
Samples from the G3PDH gene fragment amplifications using the subtracted and unsubtracted cDNA pools were analyzed after 15, 20, 25 and 30 cycles of PCR.
On the basis of the number of PCR cycles required for equal amplification of the corresponding PCR products from the subtracted and unsubtracted cDNA samples, we estimated that the subtracted libraries were 32-64-fold enriched for differentially expressed genes.
Although selecting clones that showed strong hybridization only with the forward subtracted pool was straightforward, it was more difficult to select differentially expressed genes when signals were detected in both the forward-subtracted and unsubtracted pools.
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