Words similar to unsubtracted
Example sentences for: unsubtracted
How can you use “unsubtracted” in a sentence? Here are some example sentences to help you improve your vocabulary:
Samples from the G3PDH gene fragment amplifications using the subtracted and unsubtracted cDNA pools were analyzed after 15, 20, 25 and 30 cycles of PCR.
Primary PCR amplifications were conducted for each tester using diluted subtracted products following the second hybridization or the diluted unsubtracted cDNA.
The four membranes for each subtracted tester were hybridized overnight at 42°C with 125 ng DIG labeled DNA diluted in 5 mL DIG Easy Hyb (25 ng/mL) from subtracted and unsubtracted template products of morphologies for each comparison.
Digoxigenin (DIG) labeled probes were created from subtracted and unsubtracted secondary PCR products using the DIG High Prime DNA Labeling and Detection Starter Kit (Roche Applied Science, Indianapolis, IN).
Binding intensities were quantified using a densitometer and differential gene expression of cloned template was confirmed when binding intensity was 5X greater when probed with labeled subtracted tester cDNA compared to the intensity of subtracted driver cDNA and a greater binding intensity when hybridized with unsubtracted tester cDNA compared to unsubtracted driver cDNA.
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