Example sentences for: unsubtracted

How can you use “unsubtracted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples from the G3PDH gene fragment amplifications using the subtracted and unsubtracted cDNA pools were analyzed after 15, 20, 25 and 30 cycles of PCR.

  • Although selecting clones that showed strong hybridization only with the forward subtracted pool was straightforward, it was more difficult to select differentially expressed genes when signals were detected in both the forward-subtracted and unsubtracted pools.

  • 2 for MJ treatment, and the amino-cyclopropane synthase gene ( ACS1 ) for the ozone treatments [ 9 19 35 ] . The genes tested showed strong amplification in the subtracted samples after 15 cycles of PCR, whereas in the unsubtracted samples the PCR product was seen only after 10 additional cycles (Figure 1).

  • Binding intensities were quantified using a densitometer and differential gene expression of cloned template was confirmed when binding intensity was 5X greater when probed with labeled subtracted tester cDNA compared to the intensity of subtracted driver cDNA and a greater binding intensity when hybridized with unsubtracted tester cDNA compared to unsubtracted driver cDNA.

  • Two PCR amplifications were performed on the subtracted and unsubtracted tester products.


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