Words similar to unsubtracted

Example sentences for: unsubtracted

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One of the main advantages of SSH is that it normalizes the cDNA abundance so that cDNAs encoded by genes that are expressed infrequently, but nonetheless differentially, can be identified readily [ 33 ] . The efficiency of normalization is illustrated in Figure 2. The more uniform distribution of hybridization intensities obtained using the subtracted cDNA probe (Figure 2c) reflects the equalization in the concentrations of individual species present at markedly different concentrations in the initial unsubtracted cDNA populations (Figure 2b).
cDNA clones for differentially expressed genes were identified by successively screening new clones first with the unsubtracted driver cDNA (Figure 2a) and tester (Figure 2b) cDNA pools as the probes, then the forward-subtracted (Figure 2c) and reverse-subtracted (Figure 2d) cDNA pools as probes.
On the basis of the number of PCR cycles required for equal amplification of the corresponding PCR products from the subtracted and unsubtracted cDNA samples, we estimated that the subtracted libraries were 32-64-fold enriched for differentially expressed genes.
The membranes were hybridized under stringent conditions with equivalent amounts of 32P-labeled probes generated from unsubtracted and subtracted cDNAs.
Primary PCR amplifications were conducted for each tester using diluted subtracted products following the second hybridization or the diluted unsubtracted cDNA.