Words similar to unsubtracted

Example sentences for: unsubtracted

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One of the main advantages of SSH is that it normalizes the cDNA abundance so that cDNAs encoded by genes that are expressed infrequently, but nonetheless differentially, can be identified readily [ 33 ] . The efficiency of normalization is illustrated in Figure 2. The more uniform distribution of hybridization intensities obtained using the subtracted cDNA probe (Figure 2c) reflects the equalization in the concentrations of individual species present at markedly different concentrations in the initial unsubtracted cDNA populations (Figure 2b).
Digoxigenin (DIG) labeled probes were created from subtracted and unsubtracted secondary PCR products using the DIG High Prime DNA Labeling and Detection Starter Kit (Roche Applied Science, Indianapolis, IN).
Although selecting clones that showed strong hybridization only with the forward subtracted pool was straightforward, it was more difficult to select differentially expressed genes when signals were detected in both the forward-subtracted and unsubtracted pools.
The membranes were hybridized under stringent conditions with equivalent amounts of 32P-labeled probes generated from unsubtracted and subtracted cDNAs.
Samples from the G3PDH gene fragment amplifications using the subtracted and unsubtracted cDNA pools were analyzed after 15, 20, 25 and 30 cycles of PCR.