Words similar to unsubtracted

Example sentences for: unsubtracted

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The membranes were hybridized under stringent conditions with equivalent amounts of 32P-labeled probes generated from unsubtracted and subtracted cDNAs.
Binding intensities were quantified using a densitometer and differential gene expression of cloned template was confirmed when binding intensity was 5X greater when probed with labeled subtracted tester cDNA compared to the intensity of subtracted driver cDNA and a greater binding intensity when hybridized with unsubtracted tester cDNA compared to unsubtracted driver cDNA.
Digoxigenin (DIG) labeled probes were created from subtracted and unsubtracted secondary PCR products using the DIG High Prime DNA Labeling and Detection Starter Kit (Roche Applied Science, Indianapolis, IN).
Figure 1shows that the G3PDH fragment is barely detectable even after 30 cycles of amplification in the subtracted sample, while it is clearly detectable in the unsubtracted sample after 20 cycles.
cDNA clones for differentially expressed genes were identified by successively screening new clones first with the unsubtracted driver cDNA (Figure 2a) and tester (Figure 2b) cDNA pools as the probes, then the forward-subtracted (Figure 2c) and reverse-subtracted (Figure 2d) cDNA pools as probes.