Words similar to unsubtracted
Example sentences for: unsubtracted
How can you use “unsubtracted” in a sentence? Here are some example sentences to help you improve your vocabulary:
One of the main advantages of SSH is that it normalizes the cDNA abundance so that cDNAs encoded by genes that are expressed infrequently, but nonetheless differentially, can be identified readily [ 33 ] . The efficiency of normalization is illustrated in Figure 2. The more uniform distribution of hybridization intensities obtained using the subtracted cDNA probe (Figure 2c) reflects the equalization in the concentrations of individual species present at markedly different concentrations in the initial unsubtracted cDNA populations (Figure 2b).
Figure 1shows that the G3PDH fragment is barely detectable even after 30 cycles of amplification in the subtracted sample, while it is clearly detectable in the unsubtracted sample after 20 cycles.
2 for MJ treatment, and the amino-cyclopropane synthase gene ( ACS1 ) for the ozone treatments [ 9 19 35 ] . The genes tested showed strong amplification in the subtracted samples after 15 cycles of PCR, whereas in the unsubtracted samples the PCR product was seen only after 10 additional cycles (Figure 1).
Digoxigenin (DIG) labeled probes were created from subtracted and unsubtracted secondary PCR products using the DIG High Prime DNA Labeling and Detection Starter Kit (Roche Applied Science, Indianapolis, IN).
cDNA clones for differentially expressed genes were identified by successively screening new clones first with the unsubtracted driver cDNA (Figure 2a) and tester (Figure 2b) cDNA pools as the probes, then the forward-subtracted (Figure 2c) and reverse-subtracted (Figure 2d) cDNA pools as probes.
Loading...