Words similar to unsubtracted

Example sentences for: unsubtracted

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Digoxigenin (DIG) labeled probes were created from subtracted and unsubtracted secondary PCR products using the DIG High Prime DNA Labeling and Detection Starter Kit (Roche Applied Science, Indianapolis, IN).
The four membranes for each subtracted tester were hybridized overnight at 42°C with 125 ng DIG labeled DNA diluted in 5 mL DIG Easy Hyb (25 ng/mL) from subtracted and unsubtracted template products of morphologies for each comparison.
Two PCR amplifications were performed on the subtracted and unsubtracted tester products.
One of the main advantages of SSH is that it normalizes the cDNA abundance so that cDNAs encoded by genes that are expressed infrequently, but nonetheless differentially, can be identified readily [ 33 ] . The efficiency of normalization is illustrated in Figure 2. The more uniform distribution of hybridization intensities obtained using the subtracted cDNA probe (Figure 2c) reflects the equalization in the concentrations of individual species present at markedly different concentrations in the initial unsubtracted cDNA populations (Figure 2b).
2 for MJ treatment, and the amino-cyclopropane synthase gene ( ACS1 ) for the ozone treatments [ 9 19 35 ] . The genes tested showed strong amplification in the subtracted samples after 15 cycles of PCR, whereas in the unsubtracted samples the PCR product was seen only after 10 additional cycles (Figure 1).