Words similar to unreacted

Example sentences for: unreacted

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Biotinylated Ad5.CMV-LacZ, which had been treated with 15 μg/ml sulfo-NHS-LC-biotin followed by the removal of unreacted biotinylated reagent, was used to determine the immobilization efficiency of biotinylated adenoviral vectors in streptavidin-coated wells.
This result is in contrast with our previous study, which showed that greater than 95% of biotinylated Ad5.CMV-LacZ, prepared under the same conditions, can be readily captured on streptavidin-coated microbeads [ 10 ] . Thus, the relatively low immobilization efficiency of biotinylated Ad5.CMV-LacZ on the surface of streptavidin-coated wells is not attributable to the absence of accessible biotin moieties on the surfaces of a fraction of viral particles or the presence of unreacted biotinylation reagent in the biotinylated Ad5.CMV-LacZ preparations.
Data presented here show that CAP remains bound to HIV-1 after removal of unreacted compound, impairing virus infectivity due to blockade of binding sites for cellular coreceptors CXCR4 and CCR5.
Media and ligands were replaced after 24 h and cells were harvested and assayed for CAT activity 24 h later: acetylated and unreacted [ 14C] chloramphenicol was excised from a thin layer chromatography plate and quantitated in a liquid scintillation counter.
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.