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Example sentences for: unpublished
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and R.J., unpublished work) to be trpAa/Ab/B/D/C/Eb/Ea , an operon organization seen in contemporary Cytophaga hutchinsonii, Desulfovibrio vulgaris and Coxiella burnetii (Figure 9).
First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.
Recently, improved analogue to digital capabilities and software acquisition has significantly improved our ability to record many more time points during the cardiac cycle, and with this we have noted slightly higher differences between peak and diastolic calcium levels (MacGowan and Koretsky, unpublished data).
In addition, Tm obtained from other tissues, such as inflamed tonsil, expressed levels of IL-2 that were similar to those in blood (Davis L, unpublished observation).
The MEGABLAST output was parsed into tab-delimited text and displayed using the graphical alignment display program Parasight (Jeff Bailey, unpublished work).
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