Words similar to unmethylated

Example sentences for: unmethylated

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The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
One of the unmethylated cell lines (SW756) that showed a complete absence of expression did not reactivate after treatment.
Genomic DNA was treated with sodium bisulphite as previously described [ 46 ] . Placental DNA treated in vitro with Sss I methyltransferase (New England Biolabs, Beverly, MA) and similarly treated normal lymphocyte DNA were used as controls for methylated and unmethylated templates, respectively.
These data thus suggest that global demethylation may not only influence the expression of methylated genes but also unmethylated genes.
Three of the methylated cell lines (C-4I, SiHa, and ME-180) and one of the three unmethylated cell lines (MS751) showed down regulated expression.