Words similar to unmethylated
Example sentences for: unmethylated
How can you use “unmethylated” in a sentence? Here are some example sentences to help you improve your vocabulary:
The primers used for methylated and unmethylated-specific PCR for genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 34 35 36 37 ] . For additional genes, we designed the following gene-specific primers for methylated (MF and MR) and unmethylated (UF and UR) sequences according to Herman et al [ 33 ] :
Three of the methylated cell lines (C-4I, SiHa, and ME-180) and one of the three unmethylated cell lines (MS751) showed down regulated expression.
Genomic DNA was treated with sodium bisulphite as previously described [ 33 ] . Placental DNA treated in vitro with Sss I methyltransferase (New England Biolabs, Beverly, MA) and similarly treated normal lymphocyte DNA were used as controls for methylated and unmethylated templates, respectively.
Promoter methylation has been well recognized as an important epigenetic change in the development of cancer [ 1 ] . Normally, CpG islands in the promoter regions of a number of genes are present in an unmethylated state [ 2 ] . Aberrant methylation of CpG islands in promoters is characteristic of several genes in cancer leading to loss of gene expression.
The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
Loading...