Words similar to unbound

Example sentences for: unbound

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Known numbers of biotinylated Ad5.CMV-LacZ particles were incubated in streptavidin-coated wells for immobilization, and the number of viral particles remaining unbound in the solution was estimated from their infectivity on D-17 cells (Fig.
Varying numbers of biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin, were applied to streptavidin-coated wells (well diameter, 0.64 cm) for immobilization, followed by the removal of unbound viral particles.
The wells were washed to remove unbound viral particles, and D-17 cells were placed directly in the wells (8 × 10 3cells per well) and cultured at 37°C for 48 h, followed by the analysis of the expression of the lac Z gene (Fig.
DNA-protein complexes and unbound DNA were then resolved on a 5% polyacrylamide gel, and the protein-bound DNA was purified and amplified.
Then, the same number of fresh biotinylated Ad5.CMV-LacZ was added to the well and incubated in the same manner, followed by the collection of unbound viral particles and washing of the wells.