Words similar to ultrafiltration

Example sentences for: ultrafiltration

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The viral particles were subjected to three rounds of ultrafiltration with ZM-500 centrifugal filtration units (molecular mass cut-off, 500 kDa; Millipore) using PBS (pH 7.4) containing 0.05% Tween 20 (PBST) as a diluent to remove non-virion-associated biotinylation reagent.
Ad5.CMV-LacZ was treated at varying concentrations (0 - 100 μg/ml) of sulfo-NHS-LC-biotin on ice for 2 h in PBS (pH 7.4), followed by the removal of non-virion-associated biotinylation reagent by ultrafiltration.
The Centricon centrifugal ultrafiltration devices were from Amicon/Millipore, Bedford, MA.
Ad5.CMV-LacZ was treated with varying concentrations (0 - 30 μg/ml) of sulfo-NHS-LC-biotin, and non-virion-associated biotinylation reagent was removed by repeated ultrafiltration.
Complex formation between PEDF and CM components was assayed by a method using ultrafiltration through membranes of 100,000 MW exclusion limit [ 14 ] . Binding reactions were performed with a given concentration of 125I-PEDF in defined or conditioned media, and incubations with gentle rotation at 4°C for 2 h.