Example sentences for: ultracentrifugation

How can you use “ultracentrifugation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Vectors were purified using two consecutive CsCl gradient ultracentrifugation, dialyzed at 4°C against sterile virus buffer, aliquoted, and stored at -80°C until use.

  • The supernatants from all three steps were pooled and subjected to ultracentrifugation at 100,000 g for 40 minutes at 4°C, and the pellet was resuspended in 25 mM Tris-pH7.

  • After ultracentrifugation, the supernatant was removed and the RNA pellets were dissolved in TE buffer [10 mM Tris-HCl (pH 8), 1 mM EDTA] and then treated with RNase-free DNase I (100 U/ml) to remove any residual DNA.

  • The Ad.TGF-β1 virus was purified using cesium chloride gradient ultracentrifugation at 154,000 g (30,000 rpm) and 4°C, and then dialyzed three times against Tris-buffered saline.

  • Pseudotyped retroviruses containing the vesicular stomatitis virus G protein are more robust, and can be concentrated more than 100-fold by ultracentrifugation without significant loss of viral infectivity.


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