Words similar to ubcs

Example sentences for: ubcs

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As Pcu3p-associated ubiquitin ligase activity was not described before, we first needed to determine which of the fourteen different ubiquitin-conjugating enzymes (UBCs) present in the fission yeast genome associate with the RING domain protein Pip1p, an essential cofactor of cullin ubiquitin ligases [ 7, 28, 29, 30].
produced recombinant UBCs used in Fig.
A cocktail was added that contained 8 μM ubiquitin, ATP regenerating system (2 mM HEPES at pH 7.4, 1 mM ATP, 30 mM creatine phosphate, 1 mM magnesium acetate, 0.15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1mM DTT), 500 nM bacterially expressed 6 × His-UBCs, 100 nM 6 × His-tagged human E1, 0.5 μM ubiquitin aldehyde in a volume of 15 ul.
Pcu3p-Myc complexes prepared by immunoprecipitation with Myc antibodies were then incubated with human E1, ATP, ubiquitin, and the recombinant UBCs that interacted with Pip1p.
Pcu3p-Myc complexes isolated from pcu3-13myc cells did not show any recognizable polyubiquitylation activity above background with any of the seven UBCs tested (Fig.