Words similar to uau

Example sentences for: uau

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Recombination within UAU1 to regenerate URA3 eliminates the Bgl II site and results in a cacet1::URA3 locus that would yield an ~5-kbp fragment upon digestion with Bgl II (Figure 1).
This analysis identified 16/25 of the Arg +transformants as CaCET1/cacet1::UAU1 heterozygotes.
Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg +and Ura +. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [ 16 ] . If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1 ) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3 ) would result in the selected Arg +Ura +phenotype with complete loss of the wild-type CaCET1 locus.
The blots were probed with a 5' specific CaCET1 fragment (probe A in Figure 1), which detects both the wild-type CaCET1 allele and the cacet1::UAU1 allele, and with a 3'-specific CaCET1 fragment (probe B in Figure 1) derived from the segment deleted during construction of the cacet1::UAU1 disruption cassette.
All 54 isolates also retained the cacet1::UAU1 allele that was present in the heterozygote (Fig.