Words similar to uau
Example sentences for: uau
How can you use “uau” in a sentence? Here are some example sentences to help you improve your vocabulary:
We found that the heterozygous CaCET1/cacet1::UAU1 strain displayed normal growth and morphology (not shown).
This analysis identified 16/25 of the Arg +transformants as CaCET1/cacet1::UAU1 heterozygotes.
Recombination rates at the UAU1 gene in the heterozygote were determined as described by Enloe et al [ 16 ] . Ura +segregants arose at a rate of 5 x 10 -5per division and Arg +Ura +segregants arose at rate of 8 x 10 -9per division.
For example, a probe specific for the 5' end of the CaCET1 gene (probe A in Figure 1) hybridized to a single 4.4 kbp Bgl II fragment after restriction digestion of total DNA from the parental diploid strain, whereas the heterozygote contained an additional 2.7-kbp fragment derived from scission at a novel Bgl II site located within the ARG4 component of the UAU1 insert of the disrupted cacet1::UAU1 allele (Figure 2A, lane P versus lane H).
Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg +and Ura +. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [ 16 ] . If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1 ) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3 ) would result in the selected Arg +Ura +phenotype with complete loss of the wild-type CaCET1 locus.
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