Words similar to uau

Example sentences for: uau

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Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg +and Ura +. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [ 16 ] . If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1 ) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3 ) would result in the selected Arg +Ura +phenotype with complete loss of the wild-type CaCET1 locus.
5-kbp fragment corresponding to cacet1::UAU1 (data not shown).
Recombination rates at the UAU1 gene in the heterozygote were determined as described by Enloe et al [ 16 ] . Ura +segregants arose at a rate of 5 x 10 -5per division and Arg +Ura +segregants arose at rate of 8 x 10 -9per division.
Correct insertion via homologous recombination into one copy of the CaCET1 gene, resulting in cacet1::UAU1 (Figure 1), was confirmed by Southern blotting of genomic DNA digested with diagnostic restriction endonucleases.
The sixteen CaCET1/cacet1::UAU1 heterozygotes were streaked to YPD agar and grown for 3 days at 30°C.