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Example sentences for: uams-
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S. aureus strain UAMS-1 (ATCC 49230) (osteomyelitis clinical isolate) was grown overnight in 5 ml of tryptic soy broth at 37°C with aeration.
S. aureus strain UAMS-1 at a MOI of 25:1, 75:1, or 250:1 was then added separately to Transwell ®cell culture chamber inserts.
TRAIL may mediate a number of physiological responses; therefore, in order to determine if TRAIL expressed by S. aureus -infected osteoblasts mediates osteoblast apoptosis, cultured normal mouse osteoblasts were infected with S. aureus strain UAMS-1 at various MOIs in the presence and absence of an anti-human TRAIL polyclonal antibody.
A recent report demonstrated that S. aureus -induced apoptosis of epithelial cells involves activation of caspases-8 and-3 [ 30 ] . To determine whether caspase-8 is activated in S. aureus -infected osteoblasts, cultured normal mouse osteoblasts were infected with S. aureus strain UAMS-1 at various MOIs.
Briefly, normal mouse osteoblasts were infected with S. aureus strain UAMS-1 at various MOI in the presence and absence of an anti-TRAIL polyclonal antibody (rabbit anti-human TRAIL/Apo2L, Cell Sciences, Inc., Norwood, MA).