Example sentences for: twice

How can you use “twice” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Prompted by the recent murder of a NYC plainclothes officer during a drug bust, the NYT has an informative take-out on undercover work, which points out that about twice a month a NYC undercover officer is shot during a buy, and five or six times a month, one is forced at gunpoint by dealers to use coke or heroin.

  • Each well was then washed twice with 3 mL of RPMI 1640/10% FCS, and then 1 mL of 0.1% (v/v) Triton X-100 (Sigma) was added to each well to lyse the macrophages.

  • A duplicate is not merely the submission of exactly the same case twice by computer or other error, but also any situation in which the same client returns to the program with the same legal problem in the same year.

  • Poisson probability calculations show also that under these conditions the infection occurs twice in 40% and three times in 20% of the hepatocytes.

  • Subconfluent cultures of NIH3T3 cells on 3.5 cm plastic culture dishes, were rinsed with PBS(+), fixed with 4% paraformaldehyde in PBS(+) at room temperature or 37°C, or fixed and/or extracted by one of the procedures listed in Table 1. For some experiments, cells after fixation were treated twice with 0.5 mg/ml NaBH 4 in PBS for 10 min at room temperature.


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