Example sentences for: trypsinized

How can you use “trypsinized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Passage 2 fibroblasts were trypsinized, suspended in the culture medium with 10% (v/v) dimethyl sulfoxide (Sigma) and stored as frozen aliquots.

  • Hybrid toxin 4109 consisting of domains I and II of Cry1Aa and domain III of Cry1Ac was constructed as previously described [ 3 ] . Force-feeding bioassays on B. mori and BBMV binding assays were conducted as described [ 4 ] . Crystal proteins were solubilized and trypsinized, and active toxins were column purified as carried out previously [ 19 ] .

  • In order to observe changes at the onset of apoptosis, only adherent cell populations were trypsinized, pelleted 5 minutes at 500 × g, and lysed in ice cold lysis buffer (10 mM Tris-HCl (pH 7.5), 1 mM MgCl 2 , 1 mM EGTA, 0.1 mM PMSF, 5 mM β-mercaptoethanol, 0.5% CHAPS, 10% glycerol) for 30 minutes at 4°C.

  • They were then trypsinized and seeded at a density of 3,000 cells per cm 2in 150-mm plates.

  • Sub-confluent (80-90%) LNCaP cells propagated in T25 flasks were washed with 5 ml of plain RPMI 1640 medium and trypsinized with 5 ml trypsin/EDTA solution (GIBCO-BRL, Gaithersburg, MD).


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