Example sentences for: trypsinized

How can you use “trypsinized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After the third passage, the type B synovial cells were trypsinized, counted, and cultured at a density of 5 × 10 5cells per well in 24-well plates with 1 ml DMEM supplemented with 10% FBS and 1% penicillin-streptomycin.

  • They were then trypsinized and seeded at a density of 3,000 cells per cm 2in 150-mm plates.

  • Hybrid toxin 4109 consisting of domains I and II of Cry1Aa and domain III of Cry1Ac was constructed as previously described [ 3 ] . Force-feeding bioassays on B. mori and BBMV binding assays were conducted as described [ 4 ] . Crystal proteins were solubilized and trypsinized, and active toxins were column purified as carried out previously [ 19 ] .

  • At the conclusion of the experiment, the cells were trypsinized and analyzed by flow cytometry on an EPICS XL (excited by 488 nm light, using a 530 ± 15 nm bandpass filter to detect the signal on FL1) to determine the percentage of cells expressing EGFP.

  • About 3 × 10 6pituitary cells infected, or not, 72 h previously with adeno-βgal or adeno-RGS3 were trypsinized, and cell membranes containing the GnRH receptors were prepared by placing the cells in a Dounce homogenizer (Kontes Co., Vineland, NJ) and disrupting them with 30 strokes of the pestle.


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