Example sentences for: trypsinized

How can you use “trypsinized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In order to observe changes at the onset of apoptosis, only adherent cell populations were trypsinized, pelleted 5 minutes at 500 × g, and lysed in ice cold lysis buffer (10 mM Tris-HCl (pH 7.5), 1 mM MgCl 2 , 1 mM EGTA, 0.1 mM PMSF, 5 mM β-mercaptoethanol, 0.5% CHAPS, 10% glycerol) for 30 minutes at 4°C.

  • In comparison to cultures that were not trypsinized on day 7 post transfection however, these cultures had fewer intact fluorescent cells and more debris (not shown).

  • The cells were then rinsed and trypsinized, and nuclei from control and treated cells were harvested.

  • Passage 2 fibroblasts were trypsinized, suspended in the culture medium with 10% (v/v) dimethyl sulfoxide (Sigma) and stored as frozen aliquots.

  • P19 cells in log phase growth were trypsinized and 10 5were seeded in quadruplicate into Falcon cell culture inserts, with or without Matrigel coating (Becton Dickinson, Franklin Lakes, NJ, 8.0 μm pore size, 1 × 10 5pores/sq.cm), in a volume of 0.2 ml, in a 24 well tissue culture plate.


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