Words similar to tropix
Example sentences for: tropix
How can you use “tropix” in a sentence? Here are some example sentences to help you improve your vocabulary:
Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
Reporter assays were performed using the Dual-light kit according to manufacturer's recommendations (Tropix).
Luciferase and β-galactosidase activities were measured using luciferase (Promega, Madison, WI) and Galacto-Light assay systems (Tropix, Bedford, MA).
To normalize data, cells were also transfected with β-Gal reporter and the β-galactosidase activities measured using the Galacto-star kit (Tropix, BM300S).
Cells were assayed for luciferase (Promega) activities 24 hours after addition of ligands and reporter expression was normalized to β-galactosidase activity (Tropix) according to the manufacturer's directions.