Words similar to trichloroacetic
Example sentences for: trichloroacetic
How can you use “trichloroacetic” in a sentence? Here are some example sentences to help you improve your vocabulary:
The coagulant fixatives, including methanol [ 8, 9], ethanol [ 10], acetone [ 11], and trichloroacetic acid [ 10, 12], denature and precipitate proteins.
The cells were washed five times in PBS, solubilized, the DNA precipitated with 10% trichloroacetic acid at 4°C, and counted in an scintillation counter.
Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.
At one, two, three, five and twelve minute time points, 10 μl aliquots were spotted on filter paper and placed in 5% wt/vol trichloroacetic acid, washed in 5% trichloroacetic acid twice, washed twice in 95% ethanol, rinsed in ether and scintillation-counted.
Bacterial cells were fractionated as previously described [ 18 ] . Briefly, bacterial cells were chilled on ice after growth, harvested by centrifugation, and washed in cold phosphate-buffered saline (PBS; [ 34 ] ). Bacterial whole cell fractions were prepared by resuspending the washed cells in cold PBS and precipitating total proteins with 10% (vol/vol) trichloroacetic acid (TCA) on ice overnight.