Words similar to trichloroacetic
Example sentences for: trichloroacetic
How can you use “trichloroacetic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Bacterial cells were fractionated as previously described [ 18 ] . Briefly, bacterial cells were chilled on ice after growth, harvested by centrifugation, and washed in cold phosphate-buffered saline (PBS; [ 34 ] ). Bacterial whole cell fractions were prepared by resuspending the washed cells in cold PBS and precipitating total proteins with 10% (vol/vol) trichloroacetic acid (TCA) on ice overnight.
Phosphorylase phosphatase activity was measured as the release of trichloroacetic acid (TCA) soluble counts from 32P-phosphorylase a according to Cohen et.
The cells were washed five times in PBS, solubilized, the DNA precipitated with 10% trichloroacetic acid at 4°C, and counted in an scintillation counter.
Reactions were incubated at 37°C for 3 min and terminated by the addition of ice-cold 5% trichloroacetic acid containing 5 mM inorganic pyrophosphate.
The resulting lysed-cell suspensions were then incubated on ice for 10 min, and protein was made to precipitate using 10% trichloroacetic acid.
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