Words similar to trichloroacetic
Example sentences for: trichloroacetic
How can you use “trichloroacetic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Total protein levels in the SDS extracts were determined by BCA assay (Pierce) after removing the reducing reagent from SDS buffer by precipitating protein from the extract with 10% trichloroacetic acid, washing in 10% trichloroacetic acid and resuspending the pellet in 0.1 N NaOH.
The resulting lysed-cell suspensions were then incubated on ice for 10 min, and protein was made to precipitate using 10% trichloroacetic acid.
Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.
At one, two, three, five and twelve minute time points, 10 μl aliquots were spotted on filter paper and placed in 5% wt/vol trichloroacetic acid, washed in 5% trichloroacetic acid twice, washed twice in 95% ethanol, rinsed in ether and scintillation-counted.
The reactions were stopped by addition of 500 μL of 10% trichloroacetic acid.