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Example sentences for: treatments
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Subsequent confirmation that both GAPDH and β-actin did not change was provided by RT-PCR, where a simple student's t -test with a predefined nominal α level of 0.05 indicated no significant differences between the experimental diets (B,C,D) and the control diet A. RT-PCR provided a means to confirm the effects of the 3 dietary treatments on 9 genes (Table 1) and the concordance between these 27 microarray and RT-PCR results was examined.
The available research regarding cognitive function and phytoestrogens suggests that large amounts of phytoestrogens, consumed as tofu, have an adverse influence on cognitive ability in men, where decreased brain weight, increased ventricular size and dementia have been reported [ 44 ] . In ovariectomized female rats, on the other hand, phytoestrogen treatments resulted in a dose-dependent improvement of VSM [ 45 ] . This improvement in cognitive ability in phytoestrogen treated females may be due in part to the increased presence of choline acetyltransferase messenger RNA in the frontal cortex, which has been shown to be associated with protection and enhancement of cognitive function [ 45 ] . Furthermore, we have shown that phytoestrogens significantly affect the brain calcium-binding protein calbindin (CALB), which acts as a buffer by binding intracellular calcium and plays an important role in mediating cell proliferation, programmed cell death (apoptosis), and neurotoxicity [ 48 49 50 51 52 ] . This neuroprotective mechanism via CALB appears to be important in neurological disorders such as Parkinson's disease and Huntington's disease [ 48 49 50 51 52 ] . Additionally, Cyclooxygenase-2 (COX-2) seems to play an important role in mediating functional neuronal maturation and responses to certain stimuli in the brain [ 53 54 55 ] . The expression of COX-2 is associated with key pathophysiologic events in Alzheimer's disease: deposition of beta-amyloid protein in neuritic plaques within the hippocampus and cortex [ 53 54 55 ] . Of particular importance to this study is the fact that COX-2 has been shown to be influenced by steroidal hormones [ 53 54 55 ] . Therefore, the expression of COX-2 in the frontal cortex may also be hormonally regulated and relevant to cognitive decline.
The Central Finland RA Database includes demographic measures, treatments, and outcomes of all patients with RA seen in the clinic since January 1993.
The health of test organisms is primarily assessed by the performance (survival, growth, and/or reproduction) of organisms in control treatments of individual tests.
We expressed the results as the percent increase in the fluorescence of treated cells over the fluorescence of control cells (no treatments), i.e., 100 × (PI treatment -PI control )/PI control )
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