Words similar to transposase
Example sentences for: transposase
How can you use “transposase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Transposon reactions consisted of 1 μl (50-150 ng) plasmid DNA isolated from Qiagen or Revprep DNA isolation robots, 1.6 μl 5× reaction buffer, 8 ng Entranceposon (Kan R), 0.4 μl MuA transposase, and deionized water to bring the final volume to 8 μl.
This homology is shared across a wide range of species and thus could have contributed to a natural transformation-mediated LGT event in P. stutzeri . This mechanism of acquisition is supported by the fact that no phage genes, transposase coding sequences, IS elements, or repeat sequences were found in the 25.
Other ear-enriched transcripts were for three different heat-shock proteins, late elongated hypocotyl gene and the mudrA transposase.
Several DNA sequences similar to the mariner element have been reported in G. tigrina [ 10 16 ] . There are about 7000 copies of this element in the genome, which appear to code for an active transposase [ 10 ] . None of these mariner elements are present in the genomes of other turbellarians, including the species closest to G. tigrina [ 10 ] . With the aid of PCR using specific primers for the planarian mariner element, we isolated and sequenced several clones from sexual and asexual planaria.
After heat inactivation of the MuA transposase, 2 μl of the reaction were used to transform 17 μl of DH5α chemically competent cells (Invitrogen) in 96-well format.
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