Words similar to transposase

Example sentences for: transposase

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Transposon reactions consisted of 1 μl (50-150 ng) plasmid DNA isolated from Qiagen or Revprep DNA isolation robots, 1.6 μl 5× reaction buffer, 8 ng Entranceposon (Kan R), 0.4 μl MuA transposase, and deionized water to bring the final volume to 8 μl.
Several DNA sequences similar to the mariner element have been reported in G. tigrina [ 10 16 ] . There are about 7000 copies of this element in the genome, which appear to code for an active transposase [ 10 ] . None of these mariner elements are present in the genomes of other turbellarians, including the species closest to G. tigrina [ 10 ] . With the aid of PCR using specific primers for the planarian mariner element, we isolated and sequenced several clones from sexual and asexual planaria.
0.2 fmoles of transposon were incubated with 5.0 μL of EZ::TN™ transposase (Epicentre Technologies) in 25% glycerol at 25°C for 30 min.
We adopted a cDNA sequencing strategy that relies on an in vitro transposon insertion system based on the MuA transposase, combined with primer walking (see Materials and methods for details).
To search for genes that might extend life span, new insertions of PdL were created on the second and third chromosomes by appropriate crosses to a strain expressing P element transposase (Figure 1).