Words similar to transposase
Example sentences for: transposase
How can you use “transposase” in a sentence? Here are some example sentences to help you improve your vocabulary:
This homology is shared across a wide range of species and thus could have contributed to a natural transformation-mediated LGT event in P. stutzeri . This mechanism of acquisition is supported by the fact that no phage genes, transposase coding sequences, IS elements, or repeat sequences were found in the 25.
Transposon reactions consisted of 1 μl (50-150 ng) plasmid DNA isolated from Qiagen or Revprep DNA isolation robots, 1.6 μl 5× reaction buffer, 8 ng Entranceposon (Kan R), 0.4 μl MuA transposase, and deionized water to bring the final volume to 8 μl.
After heat inactivation of the MuA transposase, 2 μl of the reaction were used to transform 17 μl of DH5α chemically competent cells (Invitrogen) in 96-well format.
The recombinant Tn 5 transposase binds these ME sequences and, in the presence of Mg 2+, catalyzes the random insertion of the transposon into target DNA in a complex process that involves generating a 9 base pair staggered nick in the target.
Several DNA sequences similar to the mariner element have been reported in G. tigrina [ 10 16 ] . There are about 7000 copies of this element in the genome, which appear to code for an active transposase [ 10 ] . None of these mariner elements are present in the genomes of other turbellarians, including the species closest to G. tigrina [ 10 ] . With the aid of PCR using specific primers for the planarian mariner element, we isolated and sequenced several clones from sexual and asexual planaria.
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