Words similar to transposase
Example sentences for: transposase
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Other ear-enriched transcripts were for three different heat-shock proteins, late elongated hypocotyl gene and the mudrA transposase.
0.2 fmoles of transposon were incubated with 5.0 μL of EZ::TN™ transposase (Epicentre Technologies) in 25% glycerol at 25°C for 30 min.
After heat inactivation of the MuA transposase, 2 μl of the reaction were used to transform 17 μl of DH5α chemically competent cells (Invitrogen) in 96-well format.
Several DNA sequences similar to the mariner element have been reported in G. tigrina [ 10 16 ] . There are about 7000 copies of this element in the genome, which appear to code for an active transposase [ 10 ] . None of these mariner elements are present in the genomes of other turbellarians, including the species closest to G. tigrina [ 10 ] . With the aid of PCR using specific primers for the planarian mariner element, we isolated and sequenced several clones from sexual and asexual planaria.
To search for genes that might extend life span, new insertions of PdL were created on the second and third chromosomes by appropriate crosses to a strain expressing P element transposase (Figure 1).