Example sentences for: transposase

How can you use “transposase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We adopted a cDNA sequencing strategy that relies on an in vitro transposon insertion system based on the MuA transposase, combined with primer walking (see Materials and methods for details).

  • This homology is shared across a wide range of species and thus could have contributed to a natural transformation-mediated LGT event in P. stutzeri . This mechanism of acquisition is supported by the fact that no phage genes, transposase coding sequences, IS elements, or repeat sequences were found in the 25.

  • The recombinant Tn 5 transposase binds these ME sequences and, in the presence of Mg 2+, catalyzes the random insertion of the transposon into target DNA in a complex process that involves generating a 9 base pair staggered nick in the target.

  • As the MudrA transposase sequence of this element, which is adjacent to the TIP1;2 sequence, is transcribed from the opposite strand, it seems that the TIP1;2 basal promoter is either extremely compact or that intra-transposon sequences serve a regulatory function.

  • After heat inactivation of the MuA transposase, 2 μl of the reaction were used to transform 17 μl of DH5α chemically competent cells (Invitrogen) in 96-well format.


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