Words similar to transposase
Example sentences for: transposase
How can you use “transposase” in a sentence? Here are some example sentences to help you improve your vocabulary:
We adopted a cDNA sequencing strategy that relies on an in vitro transposon insertion system based on the MuA transposase, combined with primer walking (see Materials and methods for details).
This homology is shared across a wide range of species and thus could have contributed to a natural transformation-mediated LGT event in P. stutzeri . This mechanism of acquisition is supported by the fact that no phage genes, transposase coding sequences, IS elements, or repeat sequences were found in the 25.
The recombinant Tn 5 transposase binds these ME sequences and, in the presence of Mg 2+, catalyzes the random insertion of the transposon into target DNA in a complex process that involves generating a 9 base pair staggered nick in the target.
As the MudrA transposase sequence of this element, which is adjacent to the TIP1;2 sequence, is transcribed from the opposite strand, it seems that the TIP1;2 basal promoter is either extremely compact or that intra-transposon sequences serve a regulatory function.
After heat inactivation of the MuA transposase, 2 μl of the reaction were used to transform 17 μl of DH5α chemically competent cells (Invitrogen) in 96-well format.