Words similar to transiently
Example sentences for: transiently
How can you use “transiently” in a sentence? Here are some example sentences to help you improve your vocabulary:
I. T. and S. S. C. carried out the studies of CAR function in transiently transfected cells.
The observation that the sur2 clone contains an integrated plasmid corresponding to the plasmid isolated from the cosmid library suggests that either a) the hybrid protein retains integrase activity, b) the plasmid inserted into the attachment site by homologous recombination between attP and attB, or c) a functional integrase was provided in trans by a second cosmid transiently present in the original transformant or by a gene present in the M. smegmatis LR222 genome.
The resulting pMIRB-HA-NPM3 plasmids (both sense and antisense orientations) were transiently transfected into NIH 3T3 cells, using transfection conditions with Lipofectamine and OptiMEM serum-free medium (Gibco-BRL, Bethesda, MD) as described [ 49 ] . Following a six-hour incubation of the DNA-Lipofectamine complexes in OptiMEM, the cells were washed and incubated in 10-cm dishes with their usual growth media (DMEM with 10% v/v fetal calf serum, 2 mM L-glutamine, 100 Units/ml of Penicillin G and 100 μg/ml Streptomycin) for 48-72 hours at 37°C in humidified 5% CO 2 incubators.
To test whether the Hh agonist could interact with Smo in vitro , we transiently overexpressed murine Smo, murine Ptc, rat β2-adrenergic receptor and GFP in 293T cells, harvested membranes and performed a filtration membrane-binding assay in a 96-well plate with [ 3H]-Hh-Ag 1.5 added at 2 nM.
The PLoS Medicine study adds more concern since it shows that although most persons can indeed transiently control their own virus, they do so for only a limited amount of time.
Loading...