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To test the effects of rexinoids on coactivator binding in a defined biochemical system, surface plasmon resonance was used to characterize the effects of the various ligands on binding of CAR, RXR and CAR/RXR heterodimers to SRC-3 (also known as ACTR, p/CIP, RAC3, AIB1, TRAM-1), an effective coactivator for CAR and many other members of the nuclear receptor superfamily.
Compared to LNCaP cells, normal male genital fibroblasts show distinctly lower baseline expression of several AR co-regulators (such as NCOA2 (GRIP-1), NCOA3 (TRAM-1), ARA54 (RNF14), data not shown).