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Example sentences for: towne
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The gB antigen used in this assay was a recombinant derivative of human CMV strain Towne gB produced as a secreted protein in Chinese hamster ovary cells [ 21 ] . The recombinant gB includes amino acids 1-676 of the extracellular domain.
To confirm the findings of Newkirk et al . [ 14 ] , we evaluated sera from individuals either naturally infected with CMV or immunized with the live attenuated Towne strain of CMV for the presence of antibodies to three antigens: Sm, RNP, and U1-70 kD.
These were preimmunization screening sera from 80 normal healthy adult females who volunteered for a Towne vaccine study.
In another study, Newkirk and coworkers also observed that a recombinant gB vaccine, which expressed the gB protein of the Towne vaccine, induced antibodies to CMV gB when administered to mice, suggesting that CMV gB induces antibodies crossreactive to U1-70 kD [ 13 ] . If this is the case, it predicts a correlation between levels of antibodies to gB and U1-70 kD in sera.
Also included were postimmunization serial sera from eight normal healthy women who had received 6000 plaque forming units of the live attenuated Towne vaccine as a single subcutaneous injection.