Example sentences for: tonoplast

How can you use “tonoplast” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In plant cells V-ATPases are responsible for the deacidification of the cytosol and the energization of secondary transport processes across the tonoplast, as well as in the endocytotic and secretory pathways [ 12 ] . In addition, the vacuolar type ATPase is thought to be primarily responsible for the acidification and expansion of the large central vacuole [ 12 13 14 15 ] . In plants a critical event in growth and development is the maturation and expansion of the central vacuole.

  • The catalytic subunit exists as two distinct genes with highly conserved exons and intron boundaries in twelve species of plants [ 25 ] . In the flowering plant Daucus carota (carrot) evidence exists for two distinct isoforms for the catalytic subunit, one of which is tonoplast specific while the other is localized to the Golgi [ 26 ] . In addition, two distinct mRNAs have been isolated from cotton ( Gossypium hirsutum ) [ 27 ] . Two subunit A mRNAs are expressed in the unicellular alga Acetabularia acetabulum [ 28 29 ] . Isoforms of other V-ATPase subunits are also known from plants including subunit B [ 27 30 32 ] , the proteolipid subunit [ 33 34 35 36 37 ] and subunits D and E [ 38 ] .

  • Three enzymes are primarily responsible for the maintenance of proton flux across the tonoplast in plants, the vacuolar H +-ATPase, the H +pumping pyrophosphatase [ 48 ] and the Na +/H +antiporter [ 49 50 ] . All three enzymes have been implicated in allowing plants to cope with environmental stress conditions.

  • The influx of water and metabolites into the vacuole is dependent in part on the generation of a proton motive force across the tonoplast.

  • An Arabidopsis CPK phosphorylates tonoplast intrinsic protein, α-TIP, a putative water-channel protein [ 76].


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