Words similar to tocopherol
Example sentences for: tocopherol
How can you use “tocopherol” in a sentence? Here are some example sentences to help you improve your vocabulary:
The adherent cells in the well were washed three times with 1.0 ml of PBS, and the cells overlaid with 400 μl of lysis buffer (1% Triton X-100 in pH 7.5, 20 mM HEPES buffer with 0.1 mM EDTA), scraped, and collected into a glass vial for tocopherol analysis by HPLC, and for protein determination by the BCA protein assay (Pierce, Rockford, IL).
Uptake is defined as the net difference between tocopherol transported into the cells and loss due to catabolism and/or in vitro oxidation.
It has been proposed that vitamin E may incorporate into cellular membranes by association of the tocopherol side chain with the polyenoic fatty acid residues in the membrane fatty acids [ 15 19 ] . This interaction may stabilize cell membranes by making highly unsaturated fatty acids less liable to peroxidation as well as by making highly unsaturated fatty acids less available for phospholipid hydrolysis by phospholipase [ 15 16 ] . The latter action may reduce mobilization of arachidonic acid (AA) from the membranes and in this way reduce the amount of AA available as a substrate to cyclooxygenase and/or lipoxygenase to produce eicosanoids with mitogenic and with inflammatory properties [ 17 20 ] . Other possible antitumor actions include: induction of apoptosis, interference with hormone production, modulation of cellular signaling and gene transcription, and induction of differentiation [ 4 21 22 23 24 ] .
The tocopherol (or tocopheryl quinone) was measured using a HPLC equipped with a Coulochem II Electrochemical Detector, a ESA Model 580 Solvent Delivery Module, a Column Catecholamine HR-80 (C18, 3 mm, 8 cm), a Model 5011 Analytical Cell, a Model 5020 Guard Cell (Chelmsford, MA), a PE Nelson 900 Series Interface with PE Nelson Turbochrom V4 software (Perkin Elmer, San Jose, CA).
The changes in tocopherol levels (expressed as nmoles/well) in the medium were also measured during both the uptake and depletion phases and these data are provided in Tables 1(for alpha-tocopherol or gamma-tocopherol) and 2(for alpha-tocopherol plus gamma-tocopherol).
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