Words similar to titrated

Example sentences for: titrated

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Sodium salts of each compound were titrated into assays of purified Fhit [ 16 ] with 1.8 μM fluorescent substrate, Appp-S-(4-4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl)methylaminoacetyl (ApppBODIPY) [ 10 ] , and competitive K i values were obtained by calculating the inhibitor concentration-dependence in reduction of k cat / K m (apparent) for substrate hydrolysis [ 10 ] . As shown in Table 1, inhibitors 2b and 3b with two-carbon diol linkers were either only as inhibitory as the natural substrate 1 or substantially less so.
Virus stocks were prepared as described previously [ 17 ] , titrated and characterized by PRA and PEA.
P1 primers were routinely titrated to determine optimum concentration [ 4 ] . This approach greatly reduces the difficulty of balancing the kinetics of biallelic ERCA amplifications.
Finally, due to the rapid decay of gene expression following maximal activation, this system can be easily titrated for the duration of the induction necessary by using slow release formulations such as ALZET mini pumps [ 18 ] . The postgenomic era has accentuated the need to develop methods to determine in vivo gene function in cancer and this ecdysone system may be a useful approach to address this.
2 1, CaCl 2 1.8, HEPES 5, and was titrated to pH 7.5 with NaOH and sterilized by filtration.