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Example sentences for: tirf

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8 μm in length [ 29 ] . Filament structures of similar size have also been observed via immunofluorescent microscopic observation of fixed cells [ 30 ] . Examination of live, GFP-myosin II expressing cells with a TIRF microscope (Fig.
Using an objective-typed TIRF microscopy, cells were imaged in an anodized aluminum imaging chamber filled with MES buffer (described above) through a coverslip with a refractive index of 1.78.
GFP-MHCK-C under the TIRF microscope displayed short particles with a longer dimension approximately half of the length of myosin II thick filaments.
This dimension is consistent with myosin II thick filaments measured from the in vitro electron microscopy photographs [ 29 ] . Using TIRF microscopy, these structures, putative filaments, are not apparent in GFP-3xAsp myosin II cells in which the three potential Thr phosphorylation targets have been replaced with Asp residues (data not shown).
Our TIRF analysis furthermore indicated that a few of the GFP-myosin II thick filaments move dynamically.