Words similar to time-resolved
Example sentences for: time-resolved
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, daidzein, genistein and equol) were determined in tissues isolated from the frontal cortex and hippocampus (brain structures critical for VSM) along with the amygdala and cerebellum brain regions, by time-resolved fluoroimmunoassay (TR-FIA) using standard methods based on previously published and validated methods for plasma and urine [ 59 60 ] . The brain tissue method (in brief) follows: The brain samples were lyophilized (pooled by treatment, n = 4 per group) from male rats used as breeders that were on the diet treatments from 50 to 120 days of age.
Time-resolved UV/vis spectroscopy methods were as described previously [ 18 ] . A Phenylsepharose™-purified pR sample was reconstituted into mixed micelles containing 1,2-diheptanoyl-SN-glycero-3-phosphocholine (DHPC), by adding a 1% solution of the short-chain lipid and then removing most of the detergent on a Sephadex G-25 column equilibrated with 1% DHPC in 100 mM NaCl.
Figure 5shows a different type of time-resolved measurement, probing not the pR chromophore, but rather pH changes in the protein environment.
BFA quantitated the time-resolved morphological alterations.
The time-resolved measurements showed no positive 400-nm absorbance signals at pH 8.0 or lower (Fig.