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Example sentences for: tigrina
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Several DNA sequences similar to the mariner element have been reported in G. tigrina [ 10 16 ] . There are about 7000 copies of this element in the genome, which appear to code for an active transposase [ 10 ] . None of these mariner elements are present in the genomes of other turbellarians, including the species closest to G. tigrina [ 10 ] . With the aid of PCR using specific primers for the planarian mariner element, we isolated and sequenced several clones from sexual and asexual planaria.
Recently, we supplemented the well-known SSH #(suppression subtractive hybridization) technology [ 7 8 ] with the Mirror Orientation Selection method (MOS) for eliminating background clones from SSH-generated libraries [ 9 ] . This innovative procedure allowed the application of these techniques in the subtraction of large eukaryotic genomes, including those of freshwater planarian (haploid genome size of G. tigrina is 1.4 × 10 9bp [ 10 ] ). Using the SSH-MOS combination, genomes of two different strains of G. tigrina were compared.
The specimens were assigned to G. tigrina on the basis of their external appearance [ 5 14 15 ] . This strain is morphologically similar to the sexual strain collected previously in Spain [ 5 ] and like the Spanish animals, differs from asexual planaria in their size and details of coloration.
We have identified a novel extrachromosomal viral-element (PEVE) in the asexual strain of planarian G. tigrina . PEVE is the first viral element identified in planaria, and comprises two potential helicases that are homologous to proteins from distant viral phyla.
Experiments were conducted on both sexual and asexual strains of the planarian Girardia (formerly Dugesia ) tigrina (Platyhelminthes, Turbellaria, Tricladida).
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