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Example sentences for: tigrina
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Recently, we supplemented the well-known SSH #(suppression subtractive hybridization) technology [ 7 8 ] with the Mirror Orientation Selection method (MOS) for eliminating background clones from SSH-generated libraries [ 9 ] . This innovative procedure allowed the application of these techniques in the subtraction of large eukaryotic genomes, including those of freshwater planarian (haploid genome size of G. tigrina is 1.4 × 10 9bp [ 10 ] ). Using the SSH-MOS combination, genomes of two different strains of G. tigrina were compared.
Experiments were conducted on both sexual and asexual strains of the planarian Girardia (formerly Dugesia ) tigrina (Platyhelminthes, Turbellaria, Tricladida).
We have identified a novel extrachromosomal viral-element (PEVE) in the asexual strain of planarian G. tigrina . PEVE is the first viral element identified in planaria, and comprises two potential helicases that are homologous to proteins from distant viral phyla.
Sequence analyses of selected AS-specific clones demonstrated that about 50% contained open reading frames (ORFs) with codon usage unusual for G. tigrina [ 17 ] . Some of these AS-specific ORFs encode polypeptides similar to viral proteins.
The specimens were assigned to G. tigrina on the basis of their external appearance [ 5 14 15 ] . This strain is morphologically similar to the sexual strain collected previously in Spain [ 5 ] and like the Spanish animals, differs from asexual planaria in their size and details of coloration.