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Example sentences for: thy-
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Briefly, specimens were washed with phosphate buffered saline (PBS) and incubated 20 minutes with mouse-anti human Thy-1, clone F10-44-1 [ 33 34 ] (5 μg/ml in PBS) reacting with placental villous pericytes, extravillous fibroblasts, and decidual cells [ 15 ] . After extensive washing in PBS, specimens were incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC, Prague, Czech Republic) diluted 1:50 and preabsorbed with rat kidney homogenate [ 15 ] . Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).
Thy-1 (F15-42-01) antibody [ 33] was kindly supplied by Dr. Rosemarie Dalchau.
1Cshows staining for Thy-1 glycoprotein, also called Thy-1 differentiation protein [ 22], of fibroblasts and pericytes associated with microvasculature (arrows) adjacent to the basement membrane.
Also, the only function of Thy-1 and other Ig-related molecules is to mediate recognition, with the consequences of recognition being due to the differentiated state of the cells.
Hence, Thy-1+ fibroblasts may stimulate differentiation and ERα expression in mononucleated trophoblast cells.
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