Words similar to thuringiensis

Example sentences for: thuringiensis

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Pulsed field gel electrophoresis was used to separate plasmid and chromosomal DNA in B. thuringiensis AWO6, and a Southern hybridization blot using a mixed pool of pXO2-derived probes (ORFs 6, 10, 50, 63, 72, 81) was performed to determine if any of the ORFs were present on the pAW63 plasmid (Figure).
Total DNA extracted from B. thuringiensis AWO6 was used to synthesize probe by incorporating [α- 32P]dCTP (6000 μCi/mMol) (NEN, Boston, MA) into randomly primed DNA synthesis reactions using the Megaprime DNA Labeling System (Amersham-Pharamacia Biotech, Piscataway, NJ) according to the manufacturer instructions.
The membrane containing B. thuringiensis AWO6 DNA was hybridized using a [α- 32P]dCTP-labeled probe prepared from a mixture of six PCR-amplified pXO2 ORF fragments (pXO2 ORFs 6, 10, 50, 63, 72, 81).
The two isolates with the most sequence conservation with pXO2 ORFs, B. thuringiensis isolates 33679 and AWO6, are known to contain large plasmids [ 12 19 ] . Four ORFs with high sequence similarity to B. thuringiensis AWO6 plasmid pAW63 were detected [ 22 ] , and a mixed pXO2 ORF probe hybridized with a PFGE fragment similar in size to pAW63.
Total genomic DNA from B. thuringiensis strain AWO6 hybridized with 23 pXO2 ORF fragments, including all ORFs tested in the region between ORF 5 and ORF 38 (Table 3).