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Example sentences for: thuringiensis

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Pulsed field gel electrophoresis was used to separate plasmid and chromosomal DNA in B. thuringiensis AWO6, and a Southern hybridization blot using a mixed pool of pXO2-derived probes (ORFs 6, 10, 50, 63, 72, 81) was performed to determine if any of the ORFs were present on the pAW63 plasmid (Figure).
B. thuringiensis AWO6 is a strain containing a 70 kb plasmid designated pAW63 [ 12 21 ] . This strain was derived from B. thuringiensis HD73 by curing of its crystal toxin bearing plasmid, pHT73 [ 12 21 ] . The pAW63 plasmid contains a replication complex that is classified as a member of the pAMB-1 family of theta replicating plasmids that are present in a broad range of Gram positive species [ 22 ] . Plasmid pXO2 also appears to be a pAMB1-like theta replicating plasmid [ 23 ] and elements surrounding the replication complex are present in both pXO2 and pAW63 (see pXO2 ORFs 35, 37, 38, 39 in Tables 2and 3).
However, two of the more distantly related isolates, B. thuringiensis 33679 and B. thuringiensis AWO6, produced 10 and 16 positive PCR reactions, respectively.
Bt, Bacillus thuringiensis ; APN, aminopeptidase N; GPI, glycosyl-phosphatidylinositol; BBMV, brush border membrane vesicles; LpNA, leucine-p-nitroanilide; PIPLC, phosphatidylinositol-specific phospholipase C; HBS, Hepes-buffered saline; SPR, surface plasmon resonance
The pXO2 ORF sequences detected in B. thuringiensis 33679 and B. thuringiensis AWO6 were distributed across the entire plasmid sequence, except in the 25.