Words similar to threonines
Example sentences for: threonines
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Myosin-II recruitment to the cortex and to the cleavage furrow cortex depends on the thick filament-state of this molecular motor [ 5 6 7 8 ] . The ability to form thick filaments is regulated by phosphorylation of three threonines near the tail of the long coiled-coil.
After purification we treated the four proteins with a combination of calcineurin and calmodulin to remove phosphates attached to serines and threonines on the recombinant proteins.
The highly conserved motif KAFSTCXSH (X is any amino acid) in IC3 contains one of the cysteines from the intracellular pair conserved in many G-protein-coupled receptors, as well as serines and threonines potentially involved in phosphorylation events as discussed below.
Substitution of the three threonines with alanines produces the 3 × Ala mutant that behaves like a constitutively unphosphorylated myosin-II.
The assembly of myosin II monomers into filaments is regulated by phosphorylation of its heavy chains at three threonine residues at the C-terminus of the tail [ 9 10 ] . Dephosphorylation of these threonines is a prerequisite of filament assembly, as confirmed by the phenotypes of a 3xAsp mutant, in which the three threonines are replaced by three aspartate residues (mimicking the phosphorylated state) [ 11 ] . In vitro the 3xAsp myosin II is severely impaired for filament assembly, and in vivo 3xAsp myosin II fails to assemble or localize to the cortical cytoskeleton.