Words similar to thiocyanate

Example sentences for: thiocyanate

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Total RNA was extracted from individual conceptuses and pools of conceptuses of identical morphologies as previously described in our laboratory [ 8 ] . Briefly, conceptuses were denatured for 15 min on ice using 500 μl of denaturing solution (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7.0, 0.5% sarcosyl, 0.1 M 2-β-mercaptoethanol), 500 μl phenol, 70 μl 2 M sodium acetate (pH 4.0), and 140 μl chloroform/iso-amyl-alcohol (49:1 fresh dilution).
The template for cDNA preparation was total RNA extracted from 10 days old larvae using the guanidine thiocyanate method.
Plasmids were introduced into strain PY967 using the lithium thiocyanate procedure [ 23 ] , and transformants were selected on SC-Ura.
Total RNA was isolated using guanidine thiocyanate extraction and 15 μg of the total RNA was analyzed by Northern hybridization as described previously [ 16 ] . cDNA probes used in hybridization were human fibronectin [ 33 ] , α1(I) collagen [ 34 ] , or cytokeratin 14 cDNA (Hung Tseng, unpublished results).
Total RNA was isolated using the guanidium thiocyanate method [ 60 ] , followed by poly A extraction using oligo dT beads [ 61 ] . Equal amounts of each RNA sample were separated on formaldehyde/agarose gels and transferred onto nitrocellulose membranes.