Words similar to thermal
Example sentences for: thermal
How can you use “thermal” in a sentence? Here are some example sentences to help you improve your vocabulary:
Thermal cycler parameters included 2 min at 50°C, 10 min at 95°C, and 40 cycles involving denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min.
Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 60 seconds, 63°C for 90 seconds for annealing, and 72°C for 90 seconds for extension.
PCRs were set up using a laboratory workstation (Biomek 1000; Beckman, Palo Alto, CA) and performed in PEC 9600 thermal cyclers (Perkin Elmer Cetus, Foster City, CA).
[ 28 ] continued this line of research by studying the evolution of thermal niche in these populations, which are maintained at a constant 37°C.
Earlier studies have shown that DAP, in DNA fragments, can widen the minor groove, as detected by reactivity towards uranyl nitrate or susceptibility to Dnase cleavage [ 29 ] , and can relieve compressions in the minor groove associated with A-tracts [ 30 ] . In contrast, NMR and molecular modeling studies of an oligonucleotide duplex containing MeC have demonstrated that, while this duplex still has a B-DNA conformation, there are differences in the structural parameters and thermal stability relative to the unmodified duplex [ 31 ] . An earlier study also found, in two closely related octanucleotide duplexes, that although the methylated duplexes retained their B-DNA conformation, different structural and thermal stability effects were seen [ 32 ] . Although these studies have been carried out with DNA-DNA and not DNA-RNA duplexes, it is possible that MeC substitution may have different structural consequences than DAP substitution.
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