Words similar to theophylline
Example sentences for: theophylline
How can you use “theophylline” in a sentence? Here are some example sentences to help you improve your vocabulary:
The effector specificity could be rationally changed by changing the specificity of the conjoined aptamer: when a mutation was introduced into the anti-theophylline aptamer that led to discrimination against theophylline and in favor of 3-methylxanthine (that also differed from theophylline by a single methyl group), the aptamer became solely responsive to 3-methylxanthine in vivo . Group I aptazymes may have a number of important biotechnology applications, including use as in vivo , real-time reporters and as regulatable gene therapeutics.
For example, the junction between the aptamer and the Group I ribozyme in the aptazyme Th2P6 resembled the deletion variant td ΔP6-6; the aptazyme was activated by theophylline, while the splicing defect of the deletion variant at 3 mM magnesium was suppressed by 8 mM magnesium or by stabilization of a tetraloop sequence that capped the P6 helix [ 15 ] . Defects that poise a ribozyme between active and inactive conformers have previously been used to engineer effector-dependence [ 20 ] .
A small aliquot (3 μl) of overnight cell culture was mixed with effector (theophylline, 7.5 mM) or H 2 O, spotted on plates, and grown overnight at 37°C.
These results were all consistent with the hypothesis that theophylline regulates intron splicing in vivo . To further probe whether this was in fact the case mRNA was isolated from E. coli treated with theophylline or not, and RT-PCR was used to confirm the presence of spliced introns (Figure 5).
The time courses reveal that the extents of splicing of the aptazymes were significantly affected by the addition of theophylline (Figures 2b,2c).
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