Words similar to theophylline
Example sentences for: theophylline
How can you use “theophylline” in a sentence? Here are some example sentences to help you improve your vocabulary:
The time courses reveal that the extents of splicing of the aptazymes were significantly affected by the addition of theophylline (Figures 2b,2c).
Splicing buffer (20 mM Tris-HCl, pH 7.5, 100 mM KCl, 3 mM MgCl 2 ), effector (theophylline, 1.5 mM) was added and the reactions were incubated on ice for an additional 15 minutes.
The ligand specificity of the anti-theophylline aptamer carried over to the theophylline-dependent aptazyme, and no activation was seen with caffeine, which differed by a single methyl group from theophylline.
The effector specificity could be rationally changed by changing the specificity of the conjoined aptamer: when a mutation was introduced into the anti-theophylline aptamer that led to discrimination against theophylline and in favor of 3-methylxanthine (that also differed from theophylline by a single methyl group), the aptamer became solely responsive to 3-methylxanthine in vivo . Group I aptazymes may have a number of important biotechnology applications, including use as in vivo , real-time reporters and as regulatable gene therapeutics.
A small aliquot (3 μl) of overnight cell culture was mixed with effector (theophylline, 7.5 mM) or H 2 O, spotted on plates, and grown overnight at 37°C.
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