Example sentences for: tfa
How can you use “tfa” in a sentence? Here are some example sentences to help you improve your vocabulary:
For analytical RP-HPLC (Reverse-phase high performance liquid chromatography Vydac C18 columns were used; the solvent system used included Water/TFA (1000:1, eluent A) and Acetonitrile/TFA (1000:1, eluent B).
The insect-selective toxin was loaded onto the C18 column and eluted in a linear gradient of 10-60 % Solvent B (95% acetonitrile; 0.1% TFA) over 60 min at a flow rate of 100 μl/min.
The individual proteins were eluted from the column with a linear gradient reaching 60% Solvent B (B= 95% acetonitrile; 0.1% TFA) in 70 min at a flow rate of 0.6 ml/min.
The freeze dried CM-fractions were resuspended in water and aliquots of 1 mg each were loaded onto the C4 reversed phase column equilibrated in Solvent A (A= 5% acetonitrile, 0.1% trifluroacetic acid, TFA).